哈茨木霉对接种尖孢镰刀菌后黄瓜根系次生代谢物的影响.pdfVIP

28(4)545-551 中国生物防治学报 Chinese Journal of Biological Control 2012 年 11 月 哈茨木霉对接种尖孢镰刀菌后黄瓜根系次生代谢物的影响 1 1* 1 1 1 2 刘爱荣 ，陈双臣 ，晋文娟 ，于贝贝 ，王凤华 ，贺超兴 （1. 河南科技大学林学院，洛阳 471003 ；2. 中国农业科学院蔬菜花卉研究所，北京 100081 ） 摘要：采用室内筛选与田间试验相结合的方法，对哈茨木霉抑制黄瓜枯萎病菌的拮抗机制进行了研究。对峙 培养结果显示木霉菌和病原菌之间形成了较明显的抑菌圈，其中菌株TN 对枯萎病菌抑制作用较强。接种木霉 菌植株显著提高了植株中肉桂醇脱氢酶（CAD ）、多酚氧化酶（PPO ）、愈创木酚过氧化物酶（G-POD ）、咖啡 酸过氧化物酶（CA-POD ）和绿原酸过氧化物酶（CGA-POD ）的活性及总酚、类黄酮、木质素的含量。接种 木霉菌可诱导根系次生代谢相关基因的上调表达，C4H、CAD、CCOMT、G6PDH、PAL 和PR-1 的表达量分 别为对照的 5.64 、5.31 、4.28 、15.33、7.36 和 6.45 倍。表明木霉菌诱导的黄瓜根部对枯萎病的抗性与植物 次生代谢密切相关。 关 键 词：哈茨木霉；拮抗作用；黄瓜枯萎病；次生代谢 中图分类号：S476.1; S436.421 文献标识码：A 文章编号：1005-9261 (2012)04-0545-07 Effects of Trichoderma harzianum on Secondary Metabolites in Cucumber Roots Infected With Fusarium oxysporum 1 1* 1 1 1 2 LIU Airong , Chen Shuangchen , Jin Wenjuan , Yu Beibei , Wang Fenghua , He Chaoxing (1. Department of Forestry, Henan University of Science and Technology, Luoyang 471003, China; 2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China) Abstract: Interaction between Trichoderma harzianum and cucumber Fusarium wilt pathogen was studied in vitro and in vivo. Three tested isolates of T. harzianum exhibited distinct inhibitory effects on Fusarium in dual tests. Isolate TN showed performed more inhibitively than the other tested isolates. Field inoculation of T. harzianum resulted in enhanced content of flavonoids, phenolics and lignin in cucumber plants. Meanwhile, the activities of CAD, PPO, G-POD, CA-POD and CGA-POD were increased by 100%, 54%, 278%, 143% an