小鼠睾丸发育过程的组织学观察1.pdfVIP

实验动物科学,2010,27(3)：10～13 小鼠睾丸发育过程的组织学观察 1 2 罗兰 张彦 杨芳 杨榆玲 （昆明医学院基础学院细胞生物学及遗传学教研室 云南 昆明，650031) 摘要：为系统观察小鼠睾丸组织发育过程，将生后 1 至 57 天处于不同发育时期的 17 组昆明种正常小鼠睾丸组织制备石蜡切片、进行 H.E 染色分析。结果表明：小鼠生后初 期睾丸曲细精管中只有支持细胞和原始生精细胞； 至生后8 天时出现B型精原细胞；15 天 时出现初级精母细胞； 23 天时出现次级精母细胞及少量圆形精子细胞，此后圆形精子细胞 逐渐增多；30 天时圆形精子细胞发生变态； 36 天时大量精子开始稳定出现于曲细精管管腔 中，并延续至此后各期。这意味着小鼠生精细胞在出生后经过一个相对连续的发育分化过程 后，至生后约 36 日发育成熟。该研究为细化小鼠睾丸组织发育和精细胞发生过程，提供了 详细资料。 关键词：小鼠，睾丸，HE 染色，组织学观察 中图分类号：Q954.43 文献标识码：A 文章编号： The histological observation in the Testis of Mice at Different Development 1 Stage 2 LUO Lan ZHANG Yan YANG Fang YANG Yuling (Dept. of Cell Biology and Genetics, Kunming Medical College, Kuming 650031) Abstract: To observe the development of testis in mice, the testis of total 17 groups of Kunming normal mice at their different developmental stages among 1-57 days after birth was performed the paraffin tissue section and HE staining. The result shows that only sertoli cells and primitive germ cells exhibit in seminiferous tubules of the early postnatal mice testis; B-type spermatogonia were observed at the 8th day after birth; Primary spermatocyte were observed at the 15th day; Secondary spermatocyte and little round spermatids appeared at the 23th day and then the quantity of round spermatids were gradually increased. At the 30th day, long-shaped sperm cells exhibited. At the 36th day, a large sum of sperms were observed in seminiferous tubules and lasted to whole subsequent stages. It means that via a process of continuously development and differentiation the primitive germ cells matured in about 36 days after birth. This study revealed more details on the development of mice testis and sperms. Key words: Mice, Testis, HE staining, histolo