体外诱导泛耐药铜绿假单胞菌与临床分离株产金属β-内酰胺酶差异分析.pdfVIP

·57· ·临床基础研究· 体外诱导泛耐药铜绿假单胞菌与临床 分离株产金属13-内酰胺酶差异分析 蔡兴俊吴华’黄奕江莫濡冰 [摘要] 目的通过建立泛耐药铜绿假单胞菌体外模型，探讨诱导株与临床分离株产金属 B-内酰胺酶的差异。方法应用多种抗菌素次抑菌浓度诱导法建立体外耐药模型，应用纸片协同 法检测诱导株与临床分离株产金属p-内酰胺酶。结果 12株I临床分离敏感株经多种抗菌素次抑 菌浓度逐步诱导，全部转为泛耐药株；应用双纸片协同法检测临床分离株产金属3-内酰胺酶比率 为52．6％(20／38)，体外诱导株为16．7％(2／12)，两组产酶率比较差异有统计学意义(P0．05)。 结论应用多种抗菌素次抑菌浓度逐步诱导法可成功建立体外耐药铜绿假单胞菌模型；次抑菌浓 度体外诱导株产金属B-内酰胺酶比率低，并非主要耐药机制。 [关键词]铜绿假单胞菌；泛耐药；次抑茵浓度；金属B．内酰胺酶 of Establishment Pseudomonasmodelinvitroanddetectionwith pandrug-resistantaeruginosa metallo-I臣-haamasee伽Xin哥un，WUHua，HUANGYiiiang，eta1．Department Medi- ofRespiratory Provincial cine，Hainan 570311．China People’sHospital。Haikou To of thedifferences betweenin— [Abstract]Objectiveexplore producingmetallo-13dactamase ducedstrainsandclinicalisolatedstrains model byestablishingpandrug．resistantpseudomonasaeruginosa in To vitro．Methodsestablish modelinvitro method drug-resistant byinducingthroushusingmultiple antibioticswith testwasusedtodetectthe ofmetal— sub．inhibitoryconcentrations；discsynergy production betweeninducedstrainsandclinicalisolatedstrmn8．Results12 lo-13-lactamases clinicalisolated．sensi． tivestrainsallconvertedtoPDRstrains methodof antibioticswith con． byinducing multiple sub．inhibitorv centrations．rIkratiosof inclinicalisolatedstrainsandinvitroinduced producingmetallo．B．1actamase strainsdetecteddouble—disctestwere by synergy 20／38(52．6％)and2／12(16．7％)，respectively，the differenceofthe ratewas betweenthesetwo enzymeproduction significant