家猪百日胚胎胸腺的基因表达谱_医学论文.docVIP

家猪百日胚胎胸腺的基因表达谱_医学论文 家猪百日胚胎胸腺的基因表达谱_医学论文 作者：陈艳炯，邓亚军，叶琳，耿佳宁，李生斌 【关键词】 猪 　　Gene expression pattern in 100day porcine foetus thymus 　　【Abstract】 AIM: To get a better understanding of the thymus development and differentiation，we constructed the gene expression pattern of 100day foetus porcine thymus(FTY). METHODS: 11712 randomly picked clones from FTY cDNA library were singlepass sequenced. After contamination was removed, 7071 cleaned ESTs were used for gene expression analysis. RESULTS: After clustering the 7071 cleaned ESTs by PHRAP, 959 contigs(sizegt1) and 3074 singletons were obtained. Blast search showed that 806 contigs and 1669 singletons (totally 5442 ESTs) had homologues in nonredundant nucleic acid database, the SwissProt database and Unigene database of human, cattle and mouse. The 1629 ESTs without similarities were considered novel. According to function, the known genes were classified into 7 group and 36.99% of ESTs were classified into the gene expression group. The thymus functional genes were expressed in a certain degree (accounting for 7.55%). Comparative expression analysis showed that the gene expression pattern of FTY was very similar to human infant thymus gene expression profile and different from that of adult human. Blast search found that 58 of 109 novel sequences with both poly A and ORF prediction had homologues to human genome sequence. CONCLUSION: EST strategy is economical and virtual in finding new genes. The 100day porcine thymus is still at a developmental stage. 　　【Keywords】 swine thymus gland/embryology EST 　　【摘要】 目的： 构建家猪100 d胚胎胸腺基因表达谱，为研究胸腺的发育和分化机制奠定基础. 方法： 随机挑取家猪100 d胸腺cDNA文库（FTY）的11 712个克隆，进行单向全自动大规模测序，去污染后共获得7071个高质量的表达序列标签(EST)用于基因表达分析. 结果： 对7071个高质量EST的聚类分析结果获得959个多拷贝基因和3074个单拷贝基因. 与非冗余的核酸数据库、SwissProt数据库及人、牛和鼠的Unigene数据库进行BLAST，发现806个多拷贝基因和1669个单拷贝基因（共计5442个ESTs）与已知序列有同源性，1629个EST没有发现同源序列被认为是新基因. 根据功能将已知基因归为7类，其中基因表达相关基因占36.99%，胸腺特异的防御功能相关基因有一定程度表达（占7.55%），这与人婴儿胸腺基因表达谱相类似而不同于成年人胸腺组织. 将109个既发现有ORF又具有PolyA的多拷贝新基因与人类基因组框架序列进行