美国红枫组织培养初步的研究.pdfVIP

美国红枫组织培养初步研究 李克壮,梅 梅,洪晓松,葛根塔娜,陆秀君 （沈阳农业大学林学院，辽宁沈阳 110866 ） 摘要：本实验以美国红枫的带芽茎段和侧芽、改良美国红枫带芽茎段为外植体，分别采用75%酒 精（10~40s）、0.1%升汞（1~6min）消毒，防褐化处理（PVP 500~700 ppm 、Vc 200ppm 、转接 至新鲜培养基），不同基本培养基（MS 、WPM ），激素种类及浓度KT （1.0~3.0mg/L）、ZT （1.0~3.0mg/L）等方面进行了研究。研究结果表明：这两种红枫硬枝带芽茎段作为外植体较优； 最佳消毒处理为酒精30s+升汞1~2min，污染率为36.7%~43.3% ；将外植体连续转接至新鲜培养基 3~5 次可彻底消除褐化；基本培养基WPM 优于MS ；ZT 的诱导率普遍高于KT ，启动培养以 WPM+ZT1.0~2.0mg/L 的效果较好，最高诱导率为91.7% 。 关键词：森林培育学；美国红枫；组织培养；外植体；褐化 A Preliminary Study on the Tissue Cultivation of Acer rubrum LI Kezhuang, MEI Mei, HONG Xiaosong, GEGEN Tana, LU Xiujun （Shenyang Agricultural University Forestry College, Shenyang, 110866, China） Abstract: This test is study in the stems with bud and lateral bud of Acer rubrum , the stems with bud of Acer ×freemanii ‘Autumn Blaze’ as explants from 75%alcohol(10~40s), 0.1%HgCl2(1~6min) sterilization, the browning control(PVP 500~700ppm 、Vc 200ppm 、transferred to fresh medium), different basal medium (MS,WPM), hormone kind and concentration KT(1.0~3.0mg/L), ZT(1.0~3.0mg/L) etc. .The results of this study are as follows: As the better explant is lignified branches (hardening) in the this two kinds of Red Maple tissue culture; Best disinfection alcohol 30s + HgCl2 2min,the contamination rate is 36.7%~43.3%; The most effective way to remove the Red Maple group training browning of explants is continuous transferred 3~5 times to fresh medium; The basal medium WPM is superior to MS; Medium supplemented with hormones ZT (zeatin) induction rate is generally higher th