基于rDNA_ITS基因齿爪金龟属三亚属系统发育关系研究.pdfVIP

() 27( 3): 220~ 223, 2010 ournal of Qingdao A gricultural University (N atural Science) : 1674- 148X(2010) 03- 0220- 04 rDNA - ITS 1 2 1 王鹏程 , 尹新路 , 顾 耘 ( 1. , 266109; 2. ) : PCR DNA , DNA ( rDNA) ( ITS1) , ( Sop hrop s Fairm aire) , : 7 ITS- 1, T, A, G, C 20. 3% 22. 9% 29. 4% 27. 4% , A+ T G+ C , (+ ) + , : ; ITS; : Q969. 516. 1 : A DO I: 10. 3969/J. ISSN. 1674- 148X. 2010. 03. 011 Research onMolecular Biology of the Three Subgenera ofH olotr ich ia Hope from rDNA - ITSGene 1 2 1 WANG Pe gche g, Y IN X i lu , GU Yu ( 1. College ofAgro omy a d P la t Protectio , QAU, Q i gdao 266109, Chi a; 2. W e de g City Agricultural Bureau) Abstract: The seque ces character of uclear ribosom e ge e ITS - 1 o the subge era ofH olotr ichia Hope w as stud ied by PCR a d DNA seque ce- detemi i atio . A s the outgroupe ofSophrop s sp. , molecular phyloge etic trees w ere established. The average co te ts ofT, A, G, C w ere 20. 3% , 22. 9% , 29. 4%, a d 27. 4%, respectively. The co te t ofA + T w as sig ifica tly low er tha that ofC+ G. The trees show ed that the relatio s of the phylige ticw ere ( H olotrichia. H olotrich ia R eitler+ H. E otr ichia M edvedev) + H. P led ina Reitle. The d ista ces amo g the three subge era ofH olotrich ia Hope w as sig ifica tly shorter tha that betw ee a yo e of the three a d the Sophrop s sp. . Key ords: H olotr ichia Hope; ITS fragme t; seque ce a alysis [ 2] (H olotrich ia Hope), ( i ter al tra 3, scribed spacer, ITS)