特异性敲除+T+细胞中+Blimp-1+对小鼠脾淋巴细胞数量及功能的影响.pdfVIP

·496· 2015 7 35 7 Chin J Microbiol Immunol， July 2015 ， Vol．35 ， No．7 · · T Blimp-1 【 】 B （Blimp-1）。 T Blimp-1 （Blimp-1CＫO）（wild-type，WT）。 ，T、B 、、 CCR7、S1P1 。 ，Blimp-1CＫO ，T、B ＋ ＋ ＋ ＋ ＋ CD4 T、CD8 T、CD19 CD5 CD1d B （ ＜0．05 ），Blimp-1CＫO ＋ ＋ ＋ ＋ CD19 CD5 B （＜0．01）。 Blimp-1CＫO CD4 T CD8 T ＋ IFN-γ、TNF-α、IL-17、IL-2 （＜0．05）。 Blimp-1CＫO CD8 T CCR7 （＜0．05），S1P1 。 Blimp-1 T 、、、，T Blimp-1 T ，B 。 【 】 B ； T ； B - - ， ， ， ， ， ， ． ， ， ， ， ， ： ，：＠． 【 】 To investigate the effects of B lymphocyte-induced maturation protein-1 （Blimp-1） on the number and function of splenic lymphocytes． The mice with defective Blimp-1 / in T cells were generated by cross-breeding B6．Blimp-1 mice with B6．Lck-Cre mice．The mononuclear lymphocytes isolated from spleen of T cell conditional Blimp-1 knockout （Blimp-1CＫO） mice and wild type ＋ ＋ （WT） C57/B6 mice were comparatively analyzed．Alterations of CD4 T and CD8 T cell subsets，the secre- tion of cytokines as well as the expression of C-C chemokine receptor type 7 （CCR7 ） and Sphingosine-1- phosphate receptor 1 （S1P1） in mice from the two groups were analyzed by flow cytometry．The changes of ＋ CD19 B cell subsets were also detected． Compared with WT mice， the total numbers of mononu- clear cells， T and B lymphocytes were all significantly increased in Blimp-1CＫO mice （＜0．05）．The ab- ＋ ＋ ＋ ＋ ＋ solute numbers of CD4 T， CD8 T and CD19 CD5 CD1d B cells in mice form Blimp-1CＫO group were higher than those of the control group （＜0．05），however，no significant differences with the percentages of