益肾消癥方对PCOS大鼠卵巢形态结构影响研究.pdf

目 录 中文摘要 ···················································1 英文摘要 ···················································3 引 言 ····················································6 正 文 ····················································7 1.实验材料和方法 ·········································7 1.1 实验材料 ·················································7 1.2 动物饲养条件 ·············································8 1.3 实验方法·················································8 1.4 统计学方法 ··············································12 2. 实验结果 ···············································12 3.讨 论 ················································16 4.存在的问题和展望 ······································38 结 论 ··················································39 参考文献 ··················································40 英文缩略名词对照 ········································45 附 图 ··················································47 综 述 ··················································50 致 谢 ··················································60 攻读学位期间发表的学术论文目录 ·······················61 作者声明 ··················································62 个人简历 ··················································63 中 文 摘 要 目 的：通过益肾消癥方对多囊卵巢综合征(polycistic ovary syndrome PCOS)大鼠卵巢形态结构影响的研究，探究益肾消癥方对PCOS 大鼠治疗机制，为进一步指导临床用药、提高临床疗效提供微观依据。 方 法 ： 选择 23 日龄体重 30-35g ，雌性清洁、健康 Sprague-Dawley(SD) 大鼠60 只, 随机分组，正常对照组15 只，模型组45 [1] 只。参照孙林,魏巍等的方法 造PCOS 大鼠模型, 37 日龄时连续阴道涂片 2 个性周期。正常对照组均符合标准，随机选取12 只，模型组中有5 只 存在规律动情周期，剔除后，随机选取36 只。模型组随机分组：模型对 照组、西药实验组、中药实验组各 12 只。中药实验组胃饲益肾消癥方； 西药实验组胃饲枸橼酸氯米芬 （CC ）；正常对照组和模型对照组大鼠同 期灌胃同体积蒸馏水。4 周后，连续阴道涂片2 个性周期。模型对照组中 1 例不符合标准者剔除后，各组随机选取 10 只。观察各组大鼠卵巢大体 解剖结构及形态结构，并进行比较分析。 结 果：1.一般状态：正常对照组大鼠皮毛紧密色白有光泽，饮 食好，活泼好动；模型对照组皮毛稀松色黄无光泽，部分皮下有散在瘀 点或瘀斑，饮食减少，喜静卧；西药实验组饮食增加，但精神、皮