《16S rRNA基因序列分析法鉴定病原细菌 Identiifcation of pathogenic microorganism by sequencing 16S rRNA gene》.pdfVIP

中南大学学报（医学版） 2013, 38(10) ; J Cent South Univ (Med Sci) 1035 DOI:10.3969/j.issn.1672-7347.2013.10.010 /xbwk/fileup/PDF/2013101035.pdf 16S rRNA基因序列分析法鉴定病原细菌 朱飞舟 ，陈利玉，陈汉春1 2 1 (中南大学 1. 生命科学学院生物化学系；2. 基础医学院微生物学系，长沙 410013) [摘要] 目的：运用16S rRNA 基因序列分析法鉴定14种细菌，为该方法的临床应用奠定基础 。方法：提取细菌 DNA，采用通用引物PCR扩增16S rRNA 基因片段并测序。将测序结果用Blastn 在线软件在Nucleotide 数据库中进行序 列同源性比对，根据序列同源性鉴定病原细菌 。结果：12种细菌可以鉴定到“种”，2种细菌可以鉴定到“属”。 结论：16S rRNA 基因序列分析是一种有效的病原细菌鉴定方法。 [关键词] 16S rRNA；病原细菌；基因序列分析 Identification of pathogenic microorganism by sequencing 16S rRNA gene 1 2 1 ZHU Feizhou , CHEN Liyu , CHEN Hanchun (1. Department of Biochemistry, School of Life Sciences; 2. Department of Microorganism, School of Basic Medicine, Central South University, Changsha 410013, China) ABSTRACT Objective: To identify 14 bacteria by sequencing the 16S rRNA gene and establish the basis for clinical application in the future. Methods: DNA samples of the 14 bacteria were extracted. The 16S rRNA genes were amplified by PCR and sequenced with common primers. The sequences of the 16S rRNA genes were aligned by online software Blastn in nucleotide database. The bacteria were identified according to the homology of their 16S rRNA genes. Results: Twelve bacteria were classified to species, the other 2 bacteria were classified to genus. Conclusion: 16S rRNA gene sequence analysis is useful in