滋补肝肾复方对CAO大鼠RhoROCK信号通路的作用研究.pdfVIP

提 要1 目的：研究滋补肝肾复方复健片对MCAO 大鼠Rho/ROCK 信号通路的作用。 方法：实验研究采用成年SPF 级雄性Sprague-Dawley （SD ）大鼠，建立MCAO 模型，分正常对照组、假手术组、模型组和药物组共四个大组，运用免疫组织化学、 Western blotting 、荧光RT-PCR 等方法从免疫病理、蛋白、基因三个水平上研究各组 大鼠缺血性脑血管病区周围脑组织中RhoA 、ROCK2 、MLCP 在造模后1 周时的表达。 结果：缺血性脑血管病后 1 周时正常对照组和假手术组大鼠脑内的 RhoA 、 ROCK2 、MLCP 呈低表达状态，二者之间无显著性差异。大鼠缺血性脑血管病后脑 内的RhoA 、ROCK2 、MLCP 表达增高，与正常对照组比较有非常显著性差异（P ＜ 0.01 ），说明缺血性脑血管病后神经再生抑制相关因子开始表达。药物组与模型组比 较显示，药物组大鼠脑组织中RhoA 、ROCK2 、MLCP 表达虽然较正常对照组增多， 但较模型组明显减少，二者之间有显著性差异（P ＜0.05 ），说明复健片可抑制RhoA 、 ROCK2 、MLCP 在缺血性脑血管病后的表达。 结论：滋补肝肾的中药复方可通过多靶点对 Rho/ROCK 信号通路的关键因子 RhoA 、ROCK2 及 MLCP 起抑制作用，以达到促进神经功能修复的临床疗效，进一 步揭示了滋补肝肾复方复健片对梗死后神经再生可能的作用机制，为中药治疗中风病 提供新的理论支持。 关键词 复健片；滋补肝肾；神经再生；Rho 1 感谢国家自然科学基金《滋补肝肾复方复健片对 MCAO大鼠Rho/ROCK 信号通路的调控作用研究》（项目编号：No对本实 验研究的资助 The Study of Mechanism of Nourishing Liver and Kidney Compound Tablet on Rho/ROCK Signal Path in MCAO Rats. Specialty: Experimental and elinical study of disease of nervous system of integratlon of traditional and weste medicine Author: Yuan Lin Yuan Lin Tutor: Professor Wang Zhonglin Professor Wang Zonglin Abstract Objective: To study the mechanism of nourishing liver and kidney compound Fujian tablet on Rho/ROCK signal path in MCAO rats. Methods: The changes of RhoA, ROCK2, MLCP were observed by immunohistochemistry, Western blotting, RT-PCR in MCAO rats, which were divided into :normal control group, sham operation group,model group,medicine group. Results: After one week, the differences of RhoA, ROCK2, MLCP were not significant between normal control group and sham operation group.The express of medicine group was higer than model group (p＜0.01 ）. The r