舒芬太尼后处理对大鼠肝缺血再灌注肾损伤的影响.docVIP

舒芬太尼后处理对大鼠肝缺血再灌注肾损伤的影响 　　【摘要】 目的：研究舒芬太尼后处理对大鼠肝缺血再灌注肾损伤的影响。方法：将Wista大鼠54只随机分为三组（n=18）：假手术组（S组），肝缺血再灌注组（I/R组），舒芬太尼后处理组（SP组）。分别于肝缺血1 h、再灌注2、4、6 h心脏穿刺采血2 mL，测定血清中胱抑素C（CysC）的含量，同时取大鼠肾组织后处死大鼠，观察大鼠肾组织的病理学变化，用免疫组化法检测Bcl-2及Bax蛋白的表达、Tunel法观察肾细胞的凋亡指数（AI）。结果：I/R组和SP组与S组相比，CysC、Bax与Bcl-2蛋白的表达及AI升高。SP组与I/R组相比，CysC、AI、Bax表达下降，Bcl-2的表达升高。结论：舒芬太尼后处理对肝缺血再灌注所造成的肾损伤的保护作用可能与Bcl-2的表达上调和Bax的表达下调有关。 　　【关键词】 肝缺血再灌注； 肾损伤； 舒芬太尼 　　Effects of Sufentanil Post-conditioning on Renal Injury Induced by Hepatic Ischemia-reperfusion in Rats/LI Min，WU Li，LIU Zhen，et al.//Medical Innovation of China，2015，12（08）：013-015 　　【Abstract】 Objective：To investigate the effects of Sufentanil post-conditioning on renal injury induced by hepatic ischemia-reperfusion in rats.Method：Fifty-four Wista rats were randomly allocated into three groups（n=18）：Sham operation group（S group），hepatic ischemia-reperfusion group（I/R group） and Sufentanil post-conditioning group（SP group）.The rats were killed at the same time to obtain the renal tissue and take blood from the heart after hepatic ischemia 1 hour reperfusion for 2，4 and 6 hours，then tested cystatinC（CysC），observed the pathological changes in renal tissue，detected the expression of Bcl-2 and Bax proteins in kindy by immunohistochemistry and the apoptosis index（AI） of the renal cells was observed by Tunel method.Result：Compared with the S group，CysC，AI and the expression of Bcl-2 and Bax proteins incresed in I/R group and SP group.Compared with the I/R group，CysC，AI and the expression of Bax protein decresed but the expression of Bcl-2 protein incresed in SP group.Conclusion：The protective effects of Sufentanil post-conditioning on renal injury induced by hepatic ischemia-reperfusion in rats may be related to the decresed expression of Bax protein and the incresed expression of Bcl-2 protein. 　　【Key words】 Hepatic ischemia-reperfusion； Renal injury； Sufentanil 　　First-author’s address：Shanxi Medical University，Taiyuan 030001，China 　　doi：10.3969/j.issn.1674-4