哮喘小鼠脾脏CD4+T细胞中microRNA.docVIP

哮喘小鼠CD4+T细胞中microRNA-31和FOXP3 mRNA的表达及相互关系的研究 酆孟洁，史菲，邱晨 （518020 广东 深圳，暨南大学第二临床医学院(深圳市人民医院) 呼吸内科） [摘要]：目的 初步探讨哮喘CD4+T细胞中microRNA-31和FOXP3的表达水平、两者的相关性及激素对它们的影响。方法 以卵蛋白（OVA）致敏激发建立哮喘小鼠模型。将15只Balb/c小鼠分为正常对照组、OVA组和地塞米松组， Elisa检测小鼠支气管肺泡灌洗液（BALF）中IL-4和IFN-γ水平；实时荧光定量PCR检测小鼠脾脏CD4+T细胞的microRNA-31和FOXP3 mRNA的水平，并分析两者之间的相关性。结果 OVA组BALF中IL-4水平高于正常对照组（228.29±66.18 pg/ml VS 66.63±17.33 pg/ml，P0.05），地塞米松组BALF中IL-4水平与正常对照组或OVA组相比，无统计学差异。各组间IFN-γ无统计学差异。OVA组CD4+T细胞中FOXP3 mRNA为正常对照组的0.10倍（P 0.05）；地塞米松组CD4+T细胞中FOXP3 mRNA为OVA组的3.75倍（P 0.05）；正常对照组与地塞米松组之间FOXP3 mRNA无统计学差异。OVA组的microRNA-31为正常对照组4.79倍（P 0.05）；地塞米松组的microRNA-31为正常对照组4.85倍（P 0.05）；OVA组microRNA-31水平和地塞米松组相比无统计学差异。microRNA-31和FOXP3 mRNA的Pearson相关系数为-0.609（P0.05）。结论 哮喘中microRNA-31和FOXP3 mRNA具有相关性。microRNA-31与地塞米松可能通过不同的途径调节CD4+T细胞中FOXP3 mRNA的表达。 [关键词] 哮喘；CD4+T细胞； microRNA -31；叉状头／翅膀状螺旋转录因子 [中图分类号] ［文献标识码］ Expression and relationship of microRNA-31 and FOXP3 mRNAs in CD4+ T cells from a mouse asthma model Feng MengJie, Shi Fei, Qiu Chen (Department of Respiratory Diseases, Shenzhen People’s Hospital, Second Clinical Medical College, Jinan University, Shenzhen, Guangdong Province, 518020, China ) ［Abstract］Objective To determine the expression levels of microRNA-31 and FOXP3 mRNAs in the spleen CD4+ T cells from asthma mice, and identify their relationship. Methods A total of 15 Balb/c mice were randomly divided into 3 groups, normal control group, model group, the dexamethasone treatment groupThe murine asthma model was sensitized and challenged by ovalbumin (OVA). The inflammatory cytokines IL-4 and IFN-γ in the bronchoalveolar lavage fluid (BALF) in the 3 groups were measured by ELISA. Expression of microRNA-31 and FOXP3 mRNAs in spleen CD4+ T cells from the 3 groups were detected by quantitative real time-PCR assay. The relationship of the expression were also analyzed. Results The BALF level of IL-4 was increased in the model group (228.29±66.18 pg/ml) compared with the n