猪油与植物黄油对脂肪组织IL―6表达影响的比较.docVIP

猪油与植物黄油对脂肪组织IL―6表达影响的比较 　　[摘要] 目的 探究猪油与植物黄油饮食对不同部位脂肪组织IL-6表达水平的影响。 方法 49只Wistar雄性大鼠，按体重随机分成3组（对照组、猪油组、植物黄油组），分别给予相应饲料喂养8周，结束时按体重增加量将实验组大鼠分为肥胖倾向组（猪油倾向组、植物黄油倾向组）与肥胖抵抗组（猪油抵抗组、植物黄油抵抗组）。比较各组大鼠体重增重、体脂肪系数以及不同部位脂肪组织IL-6表达水平。 结果 猪油和植物黄油高脂饲料致肥胖倾向大鼠体重增重明显（P 0.01）；猪油和植物黄油高脂饲料都使大鼠肾周脂肪蓄积显著多于对照组（均P 0.01）；除猪油抵抗组外，其余各组大鼠附睾周脂肪蓄积均较对照组明显增多（均P 0.01）；植物黄油喂养的大鼠皮下脂肪组织IL-6表达水平较对照组显著升高（P 0.05或P 0.01），除植物黄油抵抗组外，其余各组大鼠附睾周脂肪组织IL-6表达增多，与对照组比较，差异有高度统计学意义（均P 0.01），两种高脂饲料均可致实验大鼠肾周脂肪组织IL-6表达水平升高（均P 0.01）。 结论 猪油/植物黄油都可以导致脂肪组织炎性状态的形成；与猪油相比，植物黄油能引起皮下、内脏（附睾周、肾周）等部位脂肪组织IL-6生成增多。 　　[关键词] 猪油；植物黄油；脂肪组织；白介素-6 　　[中图分类号] R3 [文献标识码] A [文章编号] 1673-7210（2016）09（c）-0004-05 　　[Abstract] Objective To compare the effects of lard and margarine on the expression of IL-6 in different adipose tissue depots. Methods 49 male Wistar rats were assigned randomly to three groups： control （CON） group， lard group and margarine group. After given the appropriate diet for eight weeks， all rats of experimental groups were divided into the obesity prone group （L-OP， M-OP） and obesity resistant group （L-OR， M-OR） at the end of the experiment according to the weight gain. Body weight gain， body fat coefficients and the expression of IL-6 in adipose tissue were measured. Results Body weight gain of the obesity prone （OP） groups were significantly improved after given high fat diet of lard/margarine （P 0.01）. Compared with CON group， the retroperitoneal adipose tissue weights of lard and margarine group were elevated （P 0.01）； the epididymal adipose tissue weights of L-OP， M-OP and M-OR groups were significantly improved besides L-OR group respectively （P 0.01）. Compared with CON group， IL-6 expressions were increased significantly in margarine groups in subcutaneous adipose tissue （P 0.05 or P 0.01）， and which were significantly higher in epididymal adipose tissue of L-OR， L-OP and M-OP groups （P 0.01）， but no significant difference was discerned in the M-OR group respectively； more IL-6 proteins were expressed in