Affect the umbilical cord blood multipotent non-hematopoietic adult stem cells separation-related factors.docVIP

 PAGE \* MERGEFORMAT 12 Affect the umbilical cord blood multipotent non-hematopoietic adult stem cells separation-related factors Authors: Yun-Tao Li, Xu Yan, Meng star, Wei Ge, Li Bridge, Sichuan, 10000 Changchun, Yu Zhen, Chang-Hong Li, Lu-Gui Qiu 【Abstract】 In order to study under the condition of low serum isolated from umbilical cord blood multipotent non-hematopoietic adult stem cell-related factors, explore ways to optimize culture conditions, through various medium, inoculation density, the first time for liquid non-hematopoietic pluripotent umbilical cord blood adult stem cells in vitro comparison, and to select the conditions for expansion of its training to carry out immunization and induction of differentiation phenotype testing. The results showed that: in low serum conditions, DMEM/F12 medium is more suitable for cord blood multipotent non-hematopoietic adult stem cells; 1 × 106/cm2 the appropriateness of the original culture inoculation density, the original generation of the first 72 hours for the first time for liquid When asked for the best exchange of medium. The selected conditions, the use of in vitro cultured cells can continue to grow, and has a good differentiation potential. Conclusion: The establishment of human umbilical cord blood multipotent non-hematopoietic adult stem cells in vitro optimization of culture conditions for the application in tissue engineering has made a new exploration. Keywords: stem cell; multipotent non-hematopoietic adult stem cells; cord blood; separation efficiency Related Factors Affecting the Isolation of Multipotent Non-hematopoietic Adult Stem Cells from Umbilical Cord Blood Abstract To investigate the related factors affecting the isolation of multipotent non-hematopoietic adult stem cells (MNASCs) from human umbilical cord blood in low serum (2%) condition, the isolation conditions were optimized and the yield of MNASCs was improved. MNASCs from human umbilical cord bloo