Determination of total flavonoids Huperzia serrata content.docVIP

 PAGE \* MERGEFORMAT 9 Determination of total flavonoids Huperzia serrata content Author: Maxie Ho Siu-chung Yangguotangxiu China [Abstract] Objective To establish Huperzia serrata in the determination of total flavonoids. Methods rutin as reference substance, using spectrophotometry at 510 nm was measured Huperzia serrata content of total flavonoids. Results of Rutin concentration of 8.16 ~ 57.12 μ g / ml within the range of absorbance values showed good linear relationship between the regression equation was A = 10.649C 0.005 57, r = 0.998 2, the average recovery was 96.88%, RSD = 2.53% . Conclusion The method is simple, rapid, accurate, and can be used as determined Huperzia serrata method of total flavonoids. [Keywords:] Huperzia serrata; total flavonoids; rutin; spectrophotometry Abstract: ObjectiveTo establish a method for determining the content of total flavonoids in Huperzia serrata.MethodsRutin as reference sample, the total flavonoids content in huperzia serrata was determined by spectrophotometry at 510nm. ResultsThe linear relationship of rutin was good from 8.16 to 57.12 mg / ml , A = 10.649C-0.005 57, r = 0.998 2.The average recovery was 96.88%, RSD% = 2.53%. ConclusionThis method is simple, rapid and accurate. It can be used for determining the content of total flavonoids in Huperzia serrata . Keywords:: Huperzia serrat; Total flavonoids; Rutin; Spectrophotometry Huperzia serrata Huperzia serrata, alias Melaleuca tower, Yizu grass, for Huperziaceae (Huperiaceae) Huperzia (Huperzia Bernh) of a medicinal fern. Huperzia serrata likes the wet, the adaptation of the ecological environment in subtropical evergreen broad-leaved forest and southern subtropical monsoon evergreen broad-leaved secondary forest and its ecosystem are widely distributed in China [1], commonly used to treat or civil fight damage, congestion swelling, pain, schizophrenia and other diseases [2]. Huperzia serrata complex composition, the present study more at home and abroa