Effects of hypoxia on bladder smooth muscle cells of connexin expression.docVIP

 PAGE \* MERGEFORMAT 14 Effects of hypoxia on bladder smooth muscle cells of connexin expression [Abstract] Objective To study the partial bladder outlet obstruction and bladder smooth muscle cells under hypoxia in the gap junction protein expression, occurrence of unstable bladder. Methods rat model of bladder instability, bladder smooth muscle cells of normal rats in vitro and caused the cell hypoxia, using RT PCR and Western blot analysis of bladder smooth muscle cells, gap junction protein. Results unstable bladder group and hypoxia group (15min, 30min, 1h) of bladder smooth muscle cells Cx40, Cx43 at the mRNA and protein levels were higher than those in model control group and hypoxic control group (P lt;0.01), in which hypoxia 15min group Cx43 mRNA and protein expression of hypoxia than 30min, hypoxia group at 1h (P lt;0.01) . Conclusion bladder partial outlet obstruction can cause smooth muscle cell gap junction protein expression in hypoxia and increased oxygen can also cause smooth muscle cell gap junction protein expression. bladder after partial outlet obstruction caused by the occurrence of bladder instability may be related to lack of bladder smooth muscle cells Oxygen has a close relationship. [Keywords:] smooth muscle cells, hypoxia, unstable bladder, Connexin, bladder outlet obstruction ABSTRACT: Objective To study the pathogenesis of unstable bladder with partial bladder outlet obstruction through the experiment based research on connexins in both unstable bladder group and cell hypoxia group. Methods Unstable bladder rat models were established and smooth muscle cells were cultured in vitro. Then cell anoxia was induced. RT PCR and Western blot were used to quantify the connexins. Results The mRNA levels of Cx40, Cx43 and the Cx43 protein expressed in the smooth muscle cells from the unstable bladder group and cell hypoxia groups were all higher than those in the control groups (the sham operation group and normoxia groups) (P lt;0.01