Efflux system and the membrane permeability of a high level of multidrug resistance of Neisseria gonorrhoeae.docVIP

 PAGE \* MERGEFORMAT 14 Efflux system and the membrane permeability of a high level of multidrug resistance of Neisseria gonorrhoeae Of: Wang Dongmei, Wang Yong, Xia Zhongdi, Tian Feng, Zou Mingxiang [Abstract] Objective To investigate the efflux system and the membrane permeability of multi-drug resistant Neisseria gonorrhoeae. Methods PCR amplification of Neisseria mtrR promoter region contains 13bp palindromic sequence including 157 base pairs, DNA fragments and sequencing, outer membrane protein extraction, the use of SDS PAGE analysis of the constitutive. Results 5 sensitive strains did not occur mtrR promoter region mutations, 3 height level of multi-drug resistant strains of mtrR promoter region 13bp palindromic sequence occurred a single A / T base deletion, and exist in the absence of outer membrane pore protein. Conclusion mtrR 13bp palindromic sequence of the promoter region of a single A / T base deletion mtrCDE caused increased expression of efflux pump and the outer membrane pore The absence or decreased expression of membrane permeability due to decreased in mediating multi-drug resistant Neisseria gonorrhoeae in a high level play a role. [Key Words] multi-drug resistant Neisseria gonorrhoeae porin mtr ABSTRACT: Objective To investigate the impact of functional enhancement of efflux pump system and reduced permeability of outer membrane on high level multiple resistant Neisseria gonorrhoeae. Methods Several high level multiple resistant isolates with erythromycin MIC = 128.0mg / L, accompanied by concurrent resistance to several antimicrobial agents, were selected. 13bp inverted sequence positioned within the mtrR promoter region were amplified by PCR and directly sequenced to detect the possible gene change. The outer membrane proteins of the strains were extracted to analyze the constitutive profiles by SDS PAGE. Results There were no gene mutations in 5 sensitive strains. All the 3 high level multiple resistant strains contained th