Extracellular molecules CD25 gene in B lymphocytes in the immune response and the significance of the changes.docVIP

 PAGE \* MERGEFORMAT 15 Extracellular molecules CD25 gene in B lymphocytes in the immune response and the significance of the changes Of: Xu Lin, Zhang Feng, Zhou Ya, Luo Junmin, Qin Ming, Xin-Sheng Yao [Abstract] Objective elements with mouse CD25 gene vaccine extracellular Balb / c mice were observed in the humoral immune response induced by changes in B lymphocytes and its significance. Methods The pcDNA3.1-CD25 extracellular eukaryotic expression plasmid at day 0, 10 days and for 20 days with the Department of intramuscular immune Balb / c mice, enzyme-linked immunosorbent assay (ELISA) detection of anti-CD25 antibody extracellular levels and subtypes, FACS detection of spleen cells changes in the proportion of B lymphocytes and cytokines IL-4, IL-10 and IFN- expression. Results Compared with the control group, CD25 extracellular domain gene groups of anti CD25 antibody in the serum gradually increased after immunization the first 30 days reached a peak; anti-CD25 antibody-based mainly IgG1; immune B cells in mouse spleen cells did not change significantly the ratio (Pgt; 0.05), but high secretion of IL-4 and IL-10 (P lt;0.05) , and low-secreting IFN- (P lt;0.05). Conclusion CD25 extracellular protein changes in immune cell function in B marked this as a follow-up in-depth study of humoral immune response in the role of T cell vaccine provides the basis of previous experiments. [Keywords:] CD25 extracellular molecules; genetic immunization; B lymphocytes; T cell vaccine ABSTRACT: ObjectiveTo evaluate the change of B lymphocytes in humoral immune response induced by DNA vaccine encoding mouse CD25 extracellular domain (CD25e). MethodsBalb / c female mice were inoculated by intramuscular injection of pcDNA3.1-CD25e on day 0, day 10 and day 20, respectively. The titers of antibody to CD25e, as well as the class and subclass of antibody, were assayed with indirect enzyme-linked immunosorbent assay (ELISA). The percentage of B cells in splenocytes an