Flavonoid naringin inhibited RANTES production of HaCaT cells.docVIP

 PAGE \* MERGEFORMAT 9 Flavonoid naringin inhibited RANTES production of HaCaT cells [Abstract] Objective: To analyze the tumor necrosis factor-@ (TNF @) and interferon-γ (IFN γ) is induced in vitro cell line HaCaT keratinocytes produce chemokines RANTES, to know whether the effect of naringin TNF @, and IFN γ on the synthesis of Induction of RANTES. Methods: TNF @, and IFN γ stimulation of cultured HaCaT cells was determined by quantitative RT PCR of mRNA expression of RANTES, the supernatant was determined in ELISA, RANTES. Results: The normal weak expression of RANTES cultured HaCaT cells, TNF @ and IFN γ stimulation of mRNA expression in HaCaT cells and secretion of RANTES was significantly increased. naringin 0.5, 0.25 mmol · L 1 can inhibit TNF @, and IFN γ-induced RANTES secretion. Conclusion: naringin inhibits TNF @, and HaCaT cells induced by IFN γ secretion of RANTES. [Keywords:] naringin, RANTES, HaCaT cells Chemokine (Chemokine) are a class of immune cells induced by specific tissues to the infiltration and accumulation of cells with chemotactic and cytokine activation. According to the mature protein in the conserved cysteine #8203;#8203;residues (C, cysteine) of the arrangement, chemokines can be divided into 4 types: CXC-type, CC type, C type and CX3C type. regulated on activation, normal T cell expressed and secreted factor (regulated upon activation normal T cell expressed and secreted factor, RANTES) are CC family members. research shows that, RANTES increased the expression level with a variety of inflammatory diseases. such as arthritis, atopic dermatitis, psoriasis, delayed type hypersensitivity, glomerular nephritis, asthma, inflammatory colitis. RANTES has been a key mediator of acute and chronic inflammation, elevated levels of its expression is a feature of inflammation [1,2]. Flavonoids have significant antioxidant, anti-inflammatory, anti-endotoxin, and its intracellular antioxidant glutathione reductase, such as certain r