Focal cerebral ischemia in rabbits Pretreatment micro guide wire model.docVIP

 PAGE \* MERGEFORMAT 17 Focal cerebral ischemia in rabbits Pretreatment micro guide wire model [Abstract] Objective: To investigate the stability of focal cerebral ischemic preconditioning in rabbit micro-guide wire method and its evaluation model. Methods: 35 healthy New Zealand white rabbits were randomly divided into 3 groups: sham operation group 5; ischemic group, 10 rats in the DSA guidance, select the micro-guide wire directly from the common carotid artery (CCA) carotid artery (ICA) inserted Tai cerebral artery (MCA) beginning part of the 4 h, reperfusion, withdraw guide wire to the common carotid artery, the pretreatment group 20, in the DSA guidance, select the micro-guide wire from the common carotid artery carotid artery to stay plugged into the beginning part of MCA 15 min, reperfusion, withdraw the guide wire to stay within the common carotid artery 15 min, so repeated 3 times, after which the micro-wire plug in the beginning part of the middle cerebral artery 4 h, complete the micro-guide wire method model of focal cerebral ischemic preconditioning in the production and use of nerve function deficit score, TTC and HE staining to evaluate the model. and conventional MRI scan lines on the model and diffusion imaging, analysis whether MRI can show the existence of ischemic preconditioning. Results: Angiography can clearly show the internal carotid artery course and its intracranial bifurcation, 8 (80%, 8 / 10 successful animal model of ischemia, 12 (60%, 12/20 animals, a successful model of ischemic preconditioning. ischemia / reperfusion can be at 3 h after T2WI, DWI showed the outside of the right hippocampus and basal ganglia high signal, DWI range greater than T2WI, in the range of 24 h T2WI and T2WI with DWI is essentially the same close range. TTC stained normal tissue will be stained red and infarcted brain tissue white non-staining and does not stain the scope and the guide wire embolization of the lesions on T2WI consistent pretreatment