Four kinds of blood deficiency mice Comparison of preparation methods.docVIP

 PAGE \* MERGEFORMAT 6 Four kinds of blood deficiency mice Comparison of preparation methods Author: Leroy Chiao Hung, Ren Lei, Zhao Shoukang [Keywords:] blood deficiency model;,, Si Wu Tang;,, cyclophosphamide;,, acetyl phenylhydrazine Abstract: Objective selection of suitable blood-deficient mouse model of manufacturing methods. Methods cyclophosphamide 250 mg / kg intraperitoneal injection; acetyl phenylhydrazine 350 mg / kg subcutaneously; cyclophosphamide 120 mg / kg acetyl-phenylhydrazine 200 mg / kg intraperitoneal injection and cyclophosphamide 160 mg / kg intraperitoneal injection of Acetophenone hydrazine 60 mg / kg subcutaneous injection of four methods of manufacture of blood-deficient model in mice, blood peripheral blood of mice was observed in mice generally changes. The results of four kinds of methods can cause different levels of mouse blood deficiency state, the application Siwutang treatment, cyclophosphamide 160 ml / kg intraperitoneal injection of acetyl phenylhydrazine 60 mg / kg subcutaneous injection in mice of model group at reduced blood deficiency states have significantly improved. Conclusion cyclophosphamide 160 mg / kg intraperitoneal injection of acetyl phenylhydrazine 60 mg / kg subcutaneous mouse model at reduced blood deficiency can best reflect the main features of traditional Chinese medicine. Keywords:: blood deficiency model; 4 Wu-Tang; cyclophosphamide; acetyl phenylhydrazine BDS is a common traditional Chinese medicine cards, blood deficiency model is used widely in Chinese medicine animal models, but there are different ways of manufacturing model, the authors first used cyclophosphamide 250 mg / kg intraperitoneal injection; acetyl phenylhydrazine 350 mg / kg subcutaneous injection; cyclophosphamide 120 mg / kg acetyl-phenylhydrazine 200 mg / kg intraperitoneal injection of three ways to create mice with blood deficiency model and a four-Wu-Tang to treat the latter two groups, the effect is not satisfactory, to be