HPLC determination of the content of indirubin in indigo.docVIP

 PAGE \* MERGEFORMAT 9 HPLC determination of the content of indirubin in indigo 【Abstract】 Objective To determine the content of indirubin in indigo method. Methods using high performance liquid chromatography with spherisorb C18 (4.6 mm × 200 mm) for the column, methanol  0.1 mol / L ammonium acetate (65:35) as mobile phase, detection wavelength was 280 nm, flow rate of 1 ml / min, column temperature was 35 ℃. The results of indirubin in indigo content of 5.98 ~ 59.8 μg / ml range of good linearity (r = 0.999 5). The average recovery rate of 99.87%, RSD 1.31%. Conclusion The method is simple and quick measurement, reliable, and can be used as Indigo in indirubin content quality control. Keywords: indirubin; indigo; high-performance liquid chromatography Abstract: ObjectiveTo establish the method of determination of indirubin from Indigo Naturalis. Methods HPLC was employed, which chromatographic column was spherisorb C18 (4.6 mm × 200 mm), mobile phase was methanol  0.1 mol / L of ammonium acetate, flow rate was 1 ml / min, detection wavelength was 280 nm and column temperature was 35 ℃. ResultsA good linear correlationship was obtained when indirubin content ranged from 5.98 μg / ml to 59.8 μg / ml (r = 0.999 5). Average recovery was 99.87%, RSD was 1.31%. ConclusionThis method is simple, rapid, reliable and suitable for assay of indirubin from Indigo Naturalis. Keywords:: Indirubin; Indigo Naturalis; HPLC Qingdai for Cruciferae Isatis Isatis indigotica, Acanthaceae Plant Ma Lan Baphicacanthus cusia, legume Indigofera Indigoferas tinctoria, Polygonum Polygonum Polygonum tinctorium and other blue leaves or stems and leaves, obtained by processing the dry blue powder or clumps, is commonly used in traditional Chinese medicine. Recent studies found that indirubin is the indigo in the anti-cancer active ingredients, the main inhibiting DNA synthesis of leukemic cells. In order to effectively control its quality, to develop