Hypoxia-inducible factor-1α in early diabetic rat corneal epithelial cells.docVIP

 PAGE \* MERGEFORMAT 23 Hypoxia-inducible factor-1α in early diabetic rat corneal epithelial cells Author: Song Hu Ping, Yan-Nian Hui, WANG Li-li, HAN Xiao-xia, Wang Haiyan 【Abstract】 Objective: To observe the hypoxia-inducible factor-1α (HIF-1α) in the early diabetic rat corneal epithelial cells in order to investigate the corneal epithelial cells in diabetic lesions and developing role. Methods: Streptozotocin (STZ) diabetic rat model produced in the mold after the success of 1,2,4,6,8,10 and 12wk take eyeball organizations to age-matched normal rats served as control group, respectively, by immunohistochemical staining and Western blotting to detect corneal epithelial cells the expression of HIF-1α in the location and expression. Results: After the success of diabetic model induced 1wk, corneal epithelial cells that are HIF-1α expression, mainly in the basal cell nucleus, 4wk, when the number of positive cells is greater than 1wk (21.80 ± 1.93 vs 15.30 ± 2.05), 6 ~ 10wk up peak (6,8, and 10wk were 49.00 ± 2.82,90.80 ± 4.23, 51.40 ± 5.21), 12wk decreased (14.40 ± 2.06). Western blotting analysis showed that HIF-1α protein expression patterns and immunohistochemical similarities, 1wk, when that is expressed, 6 ~ 10wk peaked, 12wk down. Conclusion: HIF-1α in the diabetic corneal epithelial cells, rather than sustained high expression of transient expression may be an early diabetic corneal epithelial cells in lesions causes. Keywords: diabetic complications 0 Introduction Diabetic keratopathy is a major complication of diabetes, its main feature is corneal epithelial cell adhesion, migration, differentiation and reduced ability to update the clinical manifestations of corneal epithelial cell defect, recurrent corneal epithelial erosions, decreased sensitivity, re-epithelization delay, damage and repair capacity of anomalies and of the trauma, ulcers and increased susceptibility to edema [1]. Corneal epithelial extracellular m