Mesangial cell phenotype changes with aging.doc

 PAGE \* MERGEFORMAT 14 Mesangial cell phenotype changes with aging Of: Zhuo Li Cai Guang Fu Bo Liu Fuyou Research [Abstract] Objective To observe the mesangial cell phenotype with increasing age and the changes of function. Methods 3,12,24 months old male Wistar rat primary cultured mesangial cells, MTT assay cell proliferation, the SA gal staining, using RT PCR and Western blot were used to detect p21WAF1/CIP1/SDI1 gene and protein expression changes, and by immunofluorescence in mesangial cells p21WAF1/CIP1/SDI1 expression and localization. Results mesangial cell proliferation gradually decreased with increasing age (P lt;0.05), SA gal staining positive rate increased gradually with increasing age (P lt;0.05), p21WAF1/CIP1/SDI1 mRNA and protein expression increased with increasing age (P lt;0.05). immunofluorescence results are shown p21WAF1/CIP1/SDI1 expressed mainly in the nucleus, the fluorescence intensity gradually increased with increasing age (P lt;0.05). Conclusion rat mesangial cells with aging in cells morphology, proliferation and expression of senescence-related change obviously, there replicative senescence phenotype. [Keywords:] mesangial cells, aging, cell phenotype, p21WAF1/CIP1/SDI1 [Abstract] Objective To investigate the characteristic of rat mesangial cells with advancing age. Methods Male Wistar rats aged of 3, 12, 24 months were used. The rat mesangial cells were processed for MTT assay and senescence associated galactosidase (SA gal) staining. The expression and location of p21WAF1/CIP1/SDI1 in mesangial cells were studied by immunofluorescence. The mRNA and protein levels of p21WAF1/CIP1/SDI1 were detected by Western blot and / or RT PCR, respectively. Results The ability of proliferation was decreased in rat mesangial cells with advancing age (P lt;0.05). The expression of senescence associated galactosidase activity was increased in mesangial cells with advancing age. Percentages of SA gal staining positive cells we