Microwave assisted extraction and gas chromatography - mass spectrometry analysis of essential oil components.doc

 PAGE \* MERGEFORMAT 14 Microwave assisted extraction and gas chromatography - mass spectrometry analysis of essential oil components Of: Wangdao Wu, in Whiskers, Chen Zhimin, Zhao Quancheng, Zhang Long [Abstract] for the first time using microwave assisted extraction and gas chromatography - mass spectrometry (GC-MS) analysis of the herb in the volatile oil. Come to the best of microwave assisted extraction conditions: extraction solvent V (ethanol): V (petroleum ether) = 2:1, herbs particle size of 0.050 ~ 0.150 mm, solid to liquid ratio of 1:40, the extraction time was 25 min.GC-MS of 88 compounds isolated and identified 74 kinds of volatile oil components, can be achieved within 40 min in good separation. relative retention time of each component relative standard deviation of less than 0.15% RSD, relative peak area RSD lt;3.0%, essential oil extraction rate of 4.419 percent. [Keywords:] herb; volatile oil; microwave assisted extraction; Analysis Herb is a traditional Chinese medicine have a higher value, is a perennial herb of the Asteraceae, mainly in Jiangsu, Hubei, Shandong and other places. Into the drying of medicinal roots, and warm, bitter sweet, non-toxic, into the stomach through, with spleen dampness, expelling wind, dispelling the role of wetlands for the treatment of clinical resistance spleen and stomach, diarrhea, edema, Fengshibitong, cold flu and other diseases. Cangzhu the chemical ingredients are volatile oils, glycosides, organic acid, protein, etc. [1]. For extraction of essential oil components extracted now use more traditional - that is steam distillation, Soxhlet extraction method [2 4], but the prevalence of these methods are time-consuming, inefficient shortcomings. In recent years, microwave assisted extraction (MAE) method to overcome these shortcomings, with less solvent consumption, extraction and high efficiency is widely used in herbal extract, is an ideal extraction technology of Chinese medicine, mainly in t