Mouse vascular smooth muscle cells in primary culture method of improvement.doc

 PAGE \* MERGEFORMAT 9 Mouse vascular smooth muscle cells in primary culture method of improvement [Abstract] Objective To establish a simple and convenient but highly efficient vascular smooth muscle cells of primary culture methods. Method modified enzyme digestion. Results With this method, cell culture passage of rapid growth, cell purity above 98%, sufficient to meet the needs of a variety of cell tests. Conclusion Compared with traditional methods of the method is simple and economic, testing a high success rate, saving material and time, and get more high purity cells. WU Jian-fang (1972 ~), female, Han nationality, membership in Qinghai, Associate Professor, Master [Keywords:] mouse vascular smooth muscle cells in primary culture Abstract Objective To create a simple and effective primary culture methods for mouse vascular smooth muscle cells (VSMC). Methods Reformed enzymatic digestion. Results VSMC was grown well and fast, the purity is more than 98% which can meet various cell tests. Conclusion Comparing with traditional methods, this approaches can save much time and more materials, also have better cell quantities and purity. Keywords: Mouse Aorta VSMC Primary cult Basic research in cardiovascular disease, the biological properties of vascular smooth muscle cells in research is an extremely important part of its right to reveal the mechanism of vascular lesions is critical. Vascular smooth muscle cells in vitro test model is commonly used in recent years, along with cell culture technology, the success rate of primary culture has increased, but there are still many problems, lack of experience often have to spend a lot of time and energy to explore, not only affect the trial process and the waste of materials, while some organizations materials are extremely expensive and difficult to obtain, so in order to improve the smooth muscle cells in primary culture of success rate, and in the limited material obtained good results, we have boldly ad