Murine cervical heterotopic heart transplantation a comparison of three surgical.doc

 PAGE \* MERGEFORMAT 13 Murine cervical heterotopic heart transplantation a comparison of three surgical [Abstract] Objective three kinds of cervical heterotopic heart transplantation in mice to compare surgical to find establish a mouse model of cervical heterotopic heart transplantation a reliable method. Methods were used to-side anastomosis, end to end anastomosis, casing method cervical heterotopic heart transplantation in mice, the three kinds of surgical comparison. The results of all three methods applied in mice to complete 30 cases of cervical heterotopic heart transplantation, end-side anastomosis surgical success rate was 63.3%, surgical time (112 ± 16) min, end to end anastomosis surgical success rate was 76.7%, operative time was (95 ± 13) min, casing France procedural success rate was 93.3%, surgical time (69 ± 8) min. Conclusion The three kinds of surgical operation in the shortest tube method, the highest success rate, can establish a stable and reliable mouse model of cervical heterotopic heart transplantation. [Keywords:] Heart transplantation mouse model approach Three methods of heterotopic cervical heart transplantation in mice Abstract: Objective To contrast three operation methods of heterotopic cervical heart transplantation in mice, and explore a stable method of establishing heterotopic heart transplantation in mice. Methods The animal model was made by end  to  side anastomosis, end  to  end anastomosis, and cannulation, the operation time, achievement ratio was contrast with each other in three groups. Results We accomplished 30 operations by each method, the achievement ratio of end  to  end anastomosis was 63.3%, the operation time was (112 ± 16) min. The achievement ratio of end  to  side anastomosis was 76.7%, the operation time was (95 ± 13) min. The achievement ratio of cannulation was 93.3%, the operation time was (69 ± 8) min. Conclusion The operation time of cannulation is the shortest, and the a