New rye ketoacid derivatives D69 D activity against breast cancer.doc

 PAGE \* MERGEFORMAT 14 New rye ketoacid derivatives D69 D activity against breast cancer Of: Yuan Jie, He Zhenjian, Zhu Xun, Wu Jue-heng [Abstract] Objective To study the secondary metabolites from marine microorganisms rye ketoacid new D69 D derivatives on human breast cancer in vivo inhibitory activity, and to explore its molecular mechanism. Method than by microscopy and MTT color assay the cytotoxicity of D69, build human breast cancer nude mouse model of D69 vivo anti-tumor activity. Results D69 on human breast cancer cell line MDA-MB-435 and ZR-75-30 has significant growth inhibition effect in a dose dependent manner. its MDA-MB-435 and ZR-75-30 cell half inhibitory concentration (IC50) was 0.031 mol / L and 0.036 mol / L, while the immortalized human mammary epithelial cells HMEC lower inhibition, IC50 was 0.686 mol / L. human breast cancer xenograft model in nude mice significantly inhibited verify D69 tumor growth in vivo. Conclusion D69 may be a potential lead compounds for breast cancer chemotherapy drugs. [Keywords:] marine microorganisms; rye ketoacid D derivatives; anti-tumor; human breast cancer; human breast cancer xenograft in nude mice Abstract: ObjectiveTo investigate the in vitro and in vivo anti-breast cancer activity of D69, a novel member of secalonic acid family, which was derived from marine commensal microorganisms.Methods The inhibitory effects of D69 on growth of breast cancer cell lines and the human mammary epithelial cell line were determined by MTT assay.In addition, the in vivo antineoplastic effect of D69 on BALB / c-nude mice bearing human breast cancer cell line MDA-MB-435 was investigated.Results The IC50 values #8203;#8203;of D69 on MDA- MB-435 and ZR-75-30 cell lines after 48 h treatment were 0.031 mol / L and 0.036 mol / L, respectively.In contrast, inhibition of the growth of the immortalized breast epithelial cells, HMEC by D69, with an IC50 of 0.686 mol / L, was far milder than that of the inhibition on hu