Of mild hypothermia on acute ischemic brain protective effect of.doc

 PAGE \* MERGEFORMAT 7 Of mild hypothermia on acute ischemic brain protective effect of [Keywords:] Mild hypothermia [Keywords:] Mild hypothermia; ischemic cerebrovascular disease; cerebral protection Cerebral ischemic disease due to the high fatality rate, high disability to become a hot research neurologist doctor, the treatment of ischemic stroke central link is how to achieve the resumption of blood flow on the basis of brain protection, and rescue penumbra. The mild hypothermia on cerebral protective effect of traumatic brain injury has been confirmed by clinical and widely used in clinical [1-3] To this end, we have adopted a method of mild hypothermia on the non-craniotomy through improved embolization to observe cerebral ischemia in different time periods excitatory amino acids localized ischemia, cerebral edema, infarct volume and the morphological changes under light microscope. The results in Table Minya has a cerebral protective effect of hypothermia. Now the study are presented below. 1 Materials and methods 1.1 Animal Grouping 90 male Wistar rats weighing 250 ~ 300g, were randomly divided into three groups: ① sham-operated group 30; ② room temperature in cerebral ischemic group 30; ③ hypothermia ischemia group 30. Animals in each group also were randomly divided into 3h, 6h, 24h a total of three groups, each group animals, the number of equal divisions. Koizumi was prepared according to bolt line, commercially available single-strand nylon thread (diameter 0.16mm) and accurately select the 2.5cm head-end coated silica gel, glue criteria: long-5mm, diameter of 0.25 ~ 0.30mm. The rats were 10% chloral hydrate 3ml (3.6mg/kg body weight) intraperitoneal injection of anesthesia, the neck incision, separation of extracranial internal carotid artery the only branch of the pterygopalatine artery ligation at the bifurcation of the pterygopalatine artery. Electrocoagulation common carotid artery and carotid artery, the external carotid artery