Physical and chemical properties of protein.docVIP

 PAGE \* MERGEFORMAT 11 Physical and chemical properties of protein Keywords:: protein Proteins are large molecules composed of amino acids compounds, as part of its amino acids with similar physical and chemical properties, such as gender ionization, isoelectric point, color development reaction, the reaction of salt, but also a part of another different from the amino acids, such as high molecular weight, colloidal nature, denatured and so on. First, colloidal nature of the protein Protein molecular weight is rather high, ranging between 10000-1000000, it has reached the molecular size of the particles 1 ~ 100nm range. Globular proteins on the surface more hydrophilic groups, with a strong role in attracting the water molecules, so that often the surface of protein molecules surrounded by multi-layers of water molecules, called hydration membrane, thereby preventing the aggregation of protein particles with each other. Compared with the low molecular weight substances, protein diffusion is slow and difficult, through semi-permeable membrane, viscosity, separation and purification of protein in the process, we can make use of the protein of this nature will be mixed with a small molecule impurities in protein solution placed in the semi - transparent film made of capsule, placed in flowing water or a suitable buffer medium and small molecule impurities are easily disclosed from the pocket to retain a relatively purified capsule proteins, this method is called dialysis (dialysis). Protein molecules in solution in certain solvents can occur when the sedimentation ultracentrifugation. Radial velocity and acceleration of protein shall be the ratio of sedimentation coefficient S. Correction solvent for water, the temperature at 20 ℃ of the sedimentation coefficient S20 * w can be calculated as: where X interface for the settlement to the shaft center distance, W is the rotor angular velocity, W2X for the centripetal acceleration, dX / dt is velocity . Units