RHD multiplex PCR method for identification of genotype.docVIP

 PAGE \* MERGEFORMAT 12 RHD multiplex PCR method for identification of genotype Of: Xu Hua, Yeshi Hui, Wang Baoyan, Liumeng Li, Wu Taizhou, HE Chen [Abstract] Objective To develop a reliable PCR method for RHD genotype of blood type. Methods RHD blood group genes according to the characteristics of the design for different RHD genotype specific primers, multiplex PCR method for the establishment, and with the commercial kit comparison. The results in this research for identification of RHD multiplex PCR method for blood group genotypes, and commercial kits have the same test results. Conclusions In this study, the multiplex PCR method is simple, accurate and economic characteristics, can be used in RHD genotyping. [Keywords:] multiple PCR; RHD blood type genotype; RHD genotyping; DEL blood ABSTRACT: ObjectiveTo establish a reliable PCR method for RHD genotypes research.MethodsBased on the characteristics of RHD gene, the specific primers were designed for different RHD genotypes to establish the multiplex PCR and compare it with commercially supplied RHD genotyping kit. ResultsThe multiplex PCR utilized for RHD genotypes was established. The results of RHD genotypes were the same between the multiplex PCR method and commercially supplied kit.ConclusionThe method of the multiplex PCR can be utilized for the study of RHD genotypes because it is convenient, accurate and economical. KEY WORDS: multiplex PCR; RHD genotype; RHD genotyping; DEL blood group In recent years, the study found some of the early serological examination of individual RHD-negative after further testing, which detects D antigen and D genes. Through further research, but also can be divided into parts D (partrial-D) and weak phenotype D ( weak-D) are two types; these special RHD blood group found that more scientific guidance on safe blood transfusion and laboratory diagnosis of hemolytic disease of newborns, there is great significance [1]. At present, RHD mainly relies on th