S in patients with acute cerebral infarction.docVIP

 PAGE \* MERGEFORMAT 2 S in patients with acute cerebral infarction Authors: Guo-Hou, Chen Jun, HU Xiu-science, I Shaozu, Hua Li [Keywords:], cerebral infarction [Keywords:] cerebral infarction; S100 protein; neuron-specific enolase; myelin basic protein; prognosis An object and method 1.1 Object Case Group: hospital inpatients with acute cerebral infarction Neurology, 50 (male 29, female 21) patients, aged 43 ~ 75 years old, are in line with the Fourth National Cerebrovascular Disease Conference on the revised diagnostic criteria and confirmed by CT. Control group: 30 (male 22, female 8), aged 40 to 68 years old, both healthy persons in our hospital. 1.2 Methods Group, 32 cases of cases the onset of the first 1, 3, 5, 7, 10, 21 venous blood were collected, and the other 18 cases in the first 1, 3, 5, 7, 10 blood collection. The control group in the blood after a medical examination. specimens collected into anticoagulant tubes, rapidly centrifuged, separated plasma, -70 ℃ refrigerator under test. S100ELISA kit purchased from Fourth Military Medical largest Physiology. NSEELISA kit purchased from Beijing Bio-Technology Co., Ltd. bonding Tektronix. MBPELISA kit purchased from Huaxi Medical University School of Basic Medical Teaching recombinant DNA. use a model 550 microplate reader PR2100 made. S100, NSE and MBP concentrations of test kit used according the instructions operation and calculation results. All the cases in the pathogenesis of the day 32 patients respectively in section 21, the head CT examination, about the size of infarct location and size. by Pullicino formula lesions were divided into large, medium and small-III. infarction in the cerebral lobes in 17 cases, 14 cases of basal ganglia, internal sac area in 9 cases, 6 cases of brain stem areas, hypothalamus of 4 cases. All stroke patients admitted to hospital the same day, 10 day for NIHSS score, of which 32 patients in NIHSS score from 21 to understand the neurological impairments. 2