Tissue-engineered anterior cruciate ligament research.docVIP

 PAGE \* MERGEFORMAT 27 Tissue-engineered anterior cruciate ligament research Anterior cruciate ligament (anterior cruciate ligament, ACL) injury is the more common knee injury. ACL poor self-repair after injury, often in need of surgical reconstruction. Regular clinical use of autologous graft, allograft and synthetic materials in ACL reconstruction. The use of autologous grafts in reconstruction of ACL, there is the source restrictions, for the District of complications and other issues; the use of allograft are potential spread of disease and the risk of immune rejection; and synthetic materials, long-term efficacy of ACL reconstruction are still controversial, limited its clinical application. The development of tissue engineering technology, ACL reconstruction of the new ideas and approaches. In this paper, on tissue engineering in recent years is the seed of ACL cells, growth factors and scaffold materials, etc. are reviewed. ACL seed cells for tissue engineering a choice of ACL tissue engineering, one of the key elements is to choose the right seed cell. ACL ligament structure and function of cells as the basic unit of culture in vitro can better maintain the ligament cell phenotype, secreting type Ⅰ, Ⅲ collagen ability, is conducive to ligament tissue reconstruction. Cooper JA et al [1] will be isolated and cultured rabbit ACL, medial collateral ligament (MCL), patellar ligament, Achilles tendon 4 fibroblasts were grown in three-dimensional woven poly-lactic acid (PLLA) ACL scaffold material, the results showed that ACL cells secrete I, Ⅲ collagen and fibronectin (fibronectin, FN) gene expression level higher than other three kinds of fibroblasts that ACL tissue engineering, as the seed cells, ACL cells may be superior to other three kinds of fibroblasts . ACL cells, the same source, but there is restriction of cell proliferation during in vitro more slowly if they can solve their rapid proliferation of the problem, may well be