Applying the mutation of Bacillus subtilis and the optimization of feather fermentation medium to improve Keratinase activity英文文献资料.docVIP

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Applying the mutation of Bacillus subtilis and the optimization of feather fermentation medium to improve Keratinase activity英文文献资料.doc

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Applying the mutation of Bacillus subtilis and the optimization of feather fermentation medium to improve Keratinase activity英文文献资料

Advances in Biological Chemistry, 2012, 2, 64-69 ABC /10.4236/abc.2012.21008 Published Online February 2012 (http://www.SciRP.org/journal/abc/) Applying the mutation of Bacillus subtilis and the optimization of feather fermentation medium to improve Keratinase activity Xin Zhang Jilin Business and Technology College, Changchun, China Email: jlsyzhangxin@ Received 7 November 2011; revised 28 December 2011; accepted 8 January 2012 ABSTRACT stuff. When the macromolecule Keratoprotein of feather meal was degraded into small molecular by Bacillus sub- tilis fermentation which was easier to digeste for animals, new amino acids, bacterial protein, growth factors, im- mune substance, and beneficial active cell were produced, which not only provided protein sources for animal grow- th, but also could provide the balanced amino acids con- tent and high nutrition value. In recent years, some researchers focused their studies on feather meal degradation using mutation strains of Ba- cillus subtilis [2]. In Reference [3], the author produced mutation strains of bacillus subtilis with high feather de- gradation ability using ultraviolet. In this paper, we conducted compound mutation to Bacillus Subtilis using ultraviolet and sodium nitrite. The mutation strains with high Keratinase activity were screened. The results showed that the Keratinase activity improved a lot than before. In the optimization research, we got the optimal fermentation medium composition, using which, the Keratinase activity reached 1.117 U/mL, improved 25.22%, and the amino acids content in the broth could reach 22.66 mg/mL. Energetic Bacillus subtilis was preliminarily isolated from feather meal selection medium experiment, which could be used to produce Keratinase. Through using the ultraviolet ray produced by ultraviolet light and the compound mutation of sodium nitrite solution, a mutated strain was produced which can yield