Cardiotrophin-1 Induces Tumor Necrosis Factor Synthesis in Human Peripheral Blood Mononuclear Cells英文文献资料.docVIP

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Cardiotrophin-1 Induces Tumor Necrosis Factor Synthesis in Human Peripheral Blood Mononuclear Cells英文文献资料.doc

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Cardiotrophin-1 Induces Tumor Necrosis Factor Synthesis in Human Peripheral Blood Mononuclear Cells英文文献资料

HindawiPublishingCorporation MediatorsofIn?ammation Volume2009,ArticleID489802,7pages doi:10.1155/2009/489802 ResearchArticle Cardiotrophin-1InducesTumorNecrosisFactorαSynthesisin HumanPeripheralBloodMononuclearCells MichaelFritzenwanger, 2 1 KatharinaMeusel, ChristianJung, MarcusFranz, 1 1 1 andHans-R.Figulla 1 ZhenhuaWang, MartinFoerster, 1 1 2 DivisionofCardiology,DepartmentofInternalMedicineI,Friedrich-Schiller-UniversityofJena, ErlangerAllee101,07740Jena,Germany DepartmentofCardiology,SecondA?liatedHospitalofFujianMedicalUniversity,ZhongshanNorthRoad34, Quanzhou,362000Fujian,Germany CorrespondenceshouldbeaddressedtoMichaelFritzenwanger,michael.fritzenwanger@med.uni-jena.de Received26May2009;Revised21August2009;Accepted24November2009 RecommendedbyCharlesLarryCampbell Chronicheartfailure(CHF)isassociatedwithelevatedconcentrationsoftumornecrosisfactor(TNF)αandcardiotrophin-1(CT- 1)andalteredperipheralbloodmononuclearcell(PBMC)function.Therefore,wetestedwhetherCT-1inducesTNFαinPBMC ofhealthyvolunteers.CT-1inducedinPBMCTNFαproteininthesupernatantandTNFαmRNAinaconcentration-andtime- dependentmannerdeterminedbyELISAandreal-timePCR,respectively.MaximalTNFαproteinwasachievedwith100ng/mL CT-1after3–6hoursandmaximalTNFαmRNAinductionafter1hour.ELISAdatawerecon?rmedusingimmuno?uorescent?ow cytometry.InhibitorstudieswithactinomycinDandbrefeldinAshowedthatbothproteinsynthesisandintracellulartransport areessentialforCT-1inducedTNFαexpression.CT-1causedadosedependentnuclearfactor(NF)κBtranslocation.Parthenolide inhibitedbothNFκBtranslocationandTNFαproteinexpressionindicatingthatNFκBseemstobenecessary.Werevealedanew mechanismforelevatedserumTNFαconcentrationsandPBMCactivationinCHFbesidesthehypothesisofPBMCactivationby bacterialtranslocationfromthegut. Copyright?2009MichaelFritzenwangeretal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheor