Protein 3D Structure Computed from Evolutionary Sequence Variation 英文参考文献.docVIP

下载本文档

4
0
约10.63万字
约 20页
2017-05-13 发布于上海
举报
版权申诉

Protein 3D Structure Computed from Evolutionary Sequence Variation 英文参考文献.doc

1、本文档共20页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Protein 3D Structure Computed from Evolutionary Sequence Variation 英文参考文献

Protein3DStructureComputedfromEvolutionary SequenceVariation DeboraS.Marks1*.,LucyJ.Colwell2.,RobertSheridan3,ThomasA.Hopf1,AndreaPagnani4 ,Riccardo Zecchina4,5,ChrisSander3 1Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, United States of America, 2MRC Laboratory of Molecular Biology, Hills Road, Cambridge,UnitedKingdom,3ComputationalBiologyCenter,MemorialSloan-KetteringCancerCenter,NewYork,NewYork,UnitedStatesofAmerica,4HumanGenetics Foundation,Torino,Italy,5PolitecnicodiTorino,Torino,Italy Abstract The evolutionary trajectory of a protein through sequence space is constrained by its function. Collections of sequence homologs recordtheoutcomes ofmillions ofevolutionaryexperiments inwhich theproteinevolvesaccording tothese constraints.Decipheringtheevolutionaryrecordheldinthesesequencesandexploitingitforpredictiveandengineering purposes presents a formidable challenge. The potential benefit of solving this challenge is amplified by the advent of inexpensivehigh-throughputgenomicsequencing.Inthispaperweaskwhetherwecaninferevolutionaryconstraintsfrom asetofsequencehomologsofaprotein.Thechallengeistodistinguishtrueco-evolutioncouplingsfromthenoisysetof observedcorrelations.Weaddressthischallengeusingamaximumentropymodeloftheproteinsequence,constrainedby thestatisticsofthemultiplesequencealignment,toinferresiduepaircouplings.Surprisingly,wefindthatthestrengthof theseinferredcouplingsisanexcellentpredictorofresidue-residueproximityinfoldedstructures.Indeed,thetop-scoring residuecouplingsaresufficientlyaccurateandwell-distributedtodefinethe3Dproteinfoldwithremarkableaccuracy.We quantifythisobservationbycomputing,fromsequencealone,all-atom3Dstructuresoffifteentestproteinsfromdifferent foldclasses,ranginginsizefrom50to260residues.,includingaG-proteincoupledreceptor.Theseblindedinferencesarede novo, i.e., they do not use homology modeling or sequence-similar fragments from known structures. The co-evolution signalsprovidesufficientinformat