Revealing Natural Relationships among Arbuscular Mycorrhizal Fungi Culture Line BEG47 Represents Diversispora epigaea, Not Glomus versiforme 英文参考文献.docVIP
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Revealing Natural Relationships among Arbuscular Mycorrhizal Fungi Culture Line BEG47 Represents Diversispora epigaea, Not Glomus versiforme 英文参考文献
RevealingNaturalRelationshipsamongArbuscular
MycorrhizalFungi:CultureLineBEG47Represents
Diversisporaepigaea,NotGlomusversiforme
ArthurSchu¨?ler1*,ManuelaKru¨ger1,ChristopherWalker2,3
1DepartmentofBiology,BiocenteroftheLudwig-Maximilian-UniversityMunich,Martinsried,Germany,2RoyalBotanicGardenEdinburgh,Edinburgh,UnitedKingdom,
3SchoolofEarthandEnvironment,UniversityofWesternAustralia,WesternAustralia,Australia
Abstract
Background: Understanding the mechanisms underlying biological phenomena, such as evolutionarily conservative trait
inheritance,ispredicatedonknowledgeofthenaturalrelationshipsamongorganisms.However,despitetheirenormous
ecological significance, many of the ubiquitous soil inhabiting and plant symbiotic arbuscular mycorrhizal fungi (AMF,
phylumGlomeromycota)areincorrectlyclassified.
Methodology/Principal Findings: Here, we focused on a frequently used model AMF registered as culture BEG47. This
fungusisadescendentoftheex-typeculture-lineageofGlomusepigaeum,whichin1983wassynonymisedwithGlomus
versiforme.Ithassincethenbeenusedas‘G.versiformeBEG47’.Weshowbymorphologicalcomparisons,basedontype
material,collected1860–61,ofG.versiformeandontypematerialandlivingex-typeculturesofG.epigaeum,thatthesetwo
AMFspeciescannotbeconspecific,andbymolecularphylogeneticsthatBEG47isamemberofthegenusDiversispora.
Conclusions: This study highlights that experimental works published during the last .25 years on an AMF named ‘G.
versiforme’or‘BEG47’refertoD.epigaea,aspeciesthatisactuallyevolutionarilyseparatedbyhundredsofmillionsofyears
from all members of the genera in the Glomerales and thus from most other commonly used AMF ‘laboratory strains’.
DetailedredescriptionssubstantiatetherenamingofG.epigaeum(BEG47)asD.epigaea,positioningitsystematicallyinthe
orderDiversisporales,thusenablinganevolutionaryunderstandingofgenetical,physiological,andecologicaltraits,relative
tothoseofotherAMF.DiversisporaepigaeaiswidelyculturedasalaboratorystrainofAMF,whereasG.versiformeappears
nott
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