Restriction Site Tiling Analysis accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays 英文参考文献.docVIP

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Restriction Site Tiling Analysis accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays 英文参考文献.doc

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Restriction Site Tiling Analysis accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays 英文参考文献

Pespeni et al. Genome Biology 2010, 11:R44 /2010/11/4/R44 METHOD Open Access Restriction Site Tiling Analysis: accurate discovery Method and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays Melissa H Pespeni*1, Thomas A Oliver1, Mollie K Manier1,2 and Stephen R Palumbi1 RestrictionAotpyfrpmepisnoeetglnyhmooeddfot.rfhpoSiteohruitchsTilingaleonscdiimsanuoAnalysisdfltaneouslothci iqnuinaidentificationntitativedividualsgeno-is Abstract High-throughput genotype data can be used to identify genes important for local adaptation in wild populations, phenotypes in lab stocks, or disease-related traits in human medicine. Here we advance microarray-based genotyping for population genomics with Restriction Site Tiling Analysis. The approach simultaneously discovers polymorphisms and provides quantitative genotype data at 10,000s of loci. It is highly accurate and free from ascertainment bias. We apply the approach to uncover genomic differentiation in the purple sea urchin. Background Atlantic salmon, common frogs, and beech trees [17-21]. These methods require little prior marker or sequence information. However, they are limited by the number of Uncovering the genetic underpinnings of adaptive evolu- tion is key to understanding the evolutionary processes that generate biodiversity [1]. The combined use of loci that can be examined (usually hundreds) and the genome scans and population genetic analyses has been applied in both model and non-model organisms to dis- cover and document the role of specific genes in adaptive evolution [2-6]. Surveys of hundreds to thousands of genome-wide markers identified from SNP databases, microarray-based SNP survey methods, or sequences focus on anonymous loci limits identification of function- ally relevant genes [22]. Genome-wide scans of genetic diversity at tens of thou- sands of loci have become more accessible for non-model study systems with the deve