SectionM真核生物的转录.pptVIP

5. phosphorylation of the polymerase CTD by TFIIH Formation of a processive RNA polymerase complex and allows the RNA Pol to leave the promoter region. The initiator transcription complex For TATA-box lacking RNA Pol II promoters, TBP is recruited to the initiator element 0verlapping the start site by some DNA-binding proteins, TBP then recruit the other transcription factors and polymerase similar to TATA box gene transcription. Thanks RNA polymerase III (RNA聚合酶Ⅲ) tRNA genes (tRNA 基因) 5S rRNA genes (5SrRNA基因) Alternative RNA Pol III promoters (可变RNA聚合酶Ⅲ启动子) RNA Pol III termination (RNA聚合酶Ⅲ的终止) Section M Transcription in Eukaryotes M3-1. RNA Pol III (RNA聚合酶Ⅲ) RNA聚合酶Ⅲ, 由至少16个亚基组成。负责5SrRNA的前体 、 tRNA 及其他 snRNA和胞质RNA的转录(RNA polymerase III, is a complex of at least 16 different subunits. RNA Pol III synthesizes the precurssors of 5S rRNA, and other snRNAs and cytosolic RNAs) Section M Transcription in Eukaryotes Promoters for RNA polymerase III May consist of bipartite sequences downstream of the startpoint, with boxA separated from either boxC or boxB. Or they may consist of separated sequences upstream of the startpoint (Oct, PSE, TATA). Section M Transcription in Eukaryotes Section M Transcription in Eukaryotes M3-2. tRNA genes (tRNA基因) tRNA 基因, 来自tRNA基因的最初转录物是成熟的RNA的前体分子。tRNA基因的转录控制区位于转录单元的转录起始位点之后。在编码tRNA的DNA中有两个高度保守的序列，即A框(5’-TGGCNNAGTGG-3’)B框(5’-GGTTCGANNCC-3’) ( tRNA genes, The initial transcripts produced from tRNA genes are precursor molecules which are processed into mature RNAs. The transcription control regions of tRNA genes lie after the transcription start site within the transcription unit. There are two highly conserved sequences within the DNA encoding the tRNA, called the A box (5’-TGGCNNAGTGG-3’) and the B box (5’-GGTTCGANNCC-3’)) Section M Transcription in Eukaryotes A box and B box also encode important sequences in the tRNA itself, the D-loop and T?C-loop. Therefore, the highly conserved sequence in tRN