沉默SATB1基因对鼻咽癌细胞CNE-2增殖、迁移、侵袭及耐药的影响.pdfVIP

The influences on proliferation, migration, invasion, and drug resistance of NPC cell line CNE-2 by SATB1 silencing Abstract Objective To investigate the role of special AT rich sequence binding protein 1(SATB1) in proliferation, migration, invasion and drug resistance of nasopharyngeal carcinoma(NPC) cell line CNE-2. Methods To detect the location of SATB1 expression in CNE-2 using cell immunofluorescence; the recombined shRNA plasmid that targeted SATB1 was constructed and transected into the CNE-2 cell to silence SATB1 expression; the SATB1 mRNA and protein expression in CNE-2 cell was examined by RT-PCR and western blot. The proliferation ability and drug resistance of cells were examined by CCK8 method; the invasion ability of cells was examined by transwell assay; the migration ability of cells was examined by wound healing test. Results 1. Cell immunofluorescence revealed that SATB1 was localized in the nuclei; 2. In transwell assay, the transmembrane numbers of parental CNE-2, control shRNA and SATB1-shRNA were 58.33 ±7.64, 34.67 ±5.03, 57.33 ±2.52, respectively, compared with control and parental group, the transmembrane number of SATB1-shRNA was reduced, the difference was significant(P0 ．05), however the difference between control and parental group was not significant(P 0.05); 3. In wound healing test, the migration distance of parental CNE-2, control shRNA and SATB1-shRNA were 95.42 ±7.37, 89.89 ±16.57 , 50.97 ±17.72, respectively, compared with control and parental group, the migration distance of SATB1-shRNA was reduced, the difference was significant(P0.05), however the difference