高效液相色谱法检测牙鲆体内的生物胺.pdfVIP

现代食品科技 Modern Food Science and Technology 2011, Vol.27, No.2 高效液相色谱法检测牙鲆体内的生物胺 赵中辉，林洪，徐杰 （中国海洋大学食品科学与工程学院，山东青岛 266003） 摘要：采用高效液相色谱（HPLC ）法测定了牙鲆体内的多种生物胺。以邻苯二甲醛(OPA)为柱后荧光衍生剂，通过Capcell Pak MG C18 色谱柱的梯度洗脱，将酪胺、腐胺、尸胺、组胺、胍丁胺、亚精胺和精胺等7 种生物胺进行良好分离。测试结果表明，7 种 生物胺在 0.05~30 mg/L 的范围内线性良好，相关系数都在 0.999 以上（n=6 ），加标平均回收率为 88.8% ～106.9%，相对标准偏差（RSD ） 为 3.0% ～9.3%，最低检出限为 1.5 µg/L。结果表明，该法快速、灵敏、重现性好，能够满足牙鲆中生物胺的检测需要。 关键词：高效液相色谱；柱后衍生；生物胺；牙鲆 文章篇号：1673-9078(2011)2-228-231 Determination of Biogenic Amines in Paralichthys olivaceus by High Performance Liquid Chromatography ZHAO Zhong-hui, LIN Hong, XU Jie (College of Food Science and Engineering, Ocean University of China, Qingdao, 266003) Abstract: The method for determination of biogenic amines in Paralichthys olivaceus by reversed-phase high performance liquid chromatography and post-column derivatization with o-Phthalaldehyde （OPA ）was developed in this paper. Seven amines (tyramine, putrescine, cadaverine, histamine, agmatine, spermidine and spermine) were well separated with this method. And the seven amines had a good linearity in the range from 0.05 to 30 mg/L, with the correlation coefficients being above 0.999 (n=6). The average recoveries and the relative standard deviations of the seven amines in spiked sample were of 88.8%-106.9% and 3.0%~9.3%, respectively. The detection limits of all amines were 1.5μg/ L. In conclusion, this method was rapid and sensitive, with high recovery and fine reproducibility, which can be used to detect biogenic amines in Paralichthys olivaceus. Key words: high performance liquid chromatography (HPLC) ；post-column derivatization ；biogenic amine ；Paralichthys olivaceus 生物胺是含氮的低分子量碱性化合物，主要包