十二烷基硫酸钠增敏荧光光谱法测定盐酸小檗碱.pdfVIP

:CHEM. ANA L.) 四)1: 10. 11973/lhjy-hx201510007 L.:J:. 1乍简报 十二皖基硫酸锅增敏荧光光谱法 测定盐酸小集碱 郭颖，李午戊，刘洋，杨连利，刘静 (咸阳师范学院化学与化工学院，咸阳 712000) 摘要:基于十二:民基硫酸纳(SDS) 对盐酸小棠碱荧光有显著增敏作用，建立了测定盐酸小柴 碱的荧光光谱法。在 pH 7.0 的 Tris←HCl 缓冲溶液中，盐酸小棠碱与 O. 01 mol • L-1 SDS 溶液 的 反应 15 min 后，体系在 530 nm 处的荧光强度达到最大。盐酸小棠碱的浓度在1. 010 mg • L-] 范围内与体系荧光强度呈线性关系，检出限(3s/走)为 0.23 mg • L-] 。方法应用于制剂中盐酸小棠 碱含量的测定，结果与药典法测定结果一致。加标回收率在 94. 9%~106%之间，测定值的相对标 准偏差(n=6)在1. 9%~3. 7%之间。 关键词:荧光光谱法;盐酸小棠碱;十二;民基硫酸纳;增敏 中图分类号: 0657.3 文献标志码:A 文章编号: 1001-4020(201日10-1382-04 Determination of Berberine Hydrochloride by Fluorescent Spectrometry with Sodium DodecyI Sulfate as Sensitizer GUOYi吨， LI Wu-wu , LIU Yang , YANG Lian- Ii, LlU Jing (αllege 01 Chemistry and Chemical Engineeri晤• Xianyang Normal University. Xianyang 712000. China) Abstract: A method of f1 uorescent spectrometry was developed for the determination of berberine hydrochloride. based on the sensitization effect of sodium dodecyl sulfate (SDS) on the f1 uorescence of berberine hydrochlorid已 In the buffer solution of Tris-HCl with pH of 7. O. berberine hydrochloride reacted with SDS solution (0.01 mol • L一1) for 15 min and the system displayed the maximum fluorenscence intensity at 530 nm. A linear relationship was found between the f1 uorescence intensity and the concentration of berberine hydrochloride in the range of 1. 0-10 mg. L-] , with detection limit (3s/k) of O. 23 mg. L一] The method was applied to the determin