刺玫叶中金丝桃苷和总黄酮含量的测定.pdfVIP

※分析检测 20日，协1.34， No.02 231 自由融事 刺玫叶中金丝桃背和总黄酣含量的测定 钟方丽，王晓林*，孙晓雨，王建刚 (吉林化工学院化学与制药工程学院，吉林吉林 132022) 摘 要z 目的t 建立测定刺政叶中金丝桃苦和总黄酣含量的方法。方法:以芦丁为对照品，采用紫外分光光 度法于418nm测定总黄酣含量:采用高效液相色谱法测定金丝桃昔含量，采用Shim-pack VP-ODS C 色谱柱 18 (150mmX4.6mm , 5阳时，流动相为甲醇-0.5%磷酸(45:55 ， V/η 溶液，检测波长为36伽un，流速为1.仇咀Jmin， 柱温 2 40C。结果2 总黄酣在0.02~0.12mglmL (R =0.9996)、金丝桃背在9.60~57.60μ，glmL(k=0.9993)质量浓度范围内呈 良好线性关系:总黄酣和金丝桃昔平均回收率分别为100.87%和1∞.36%， RSD分别为2.79%和2.26%。结论z 所建 立的方法快速、准确、操作简便，适用于刺玫叶中金丝挑昔和总黄酣的含量测定。 关键词:刺玫叶:总黄酣:金丝桃昔:芦丁:高效液相法:紫外分光光度法 Detennination of Hyperoside and Total Flavonoids in Leaves ofRosa davurica Pall. ZHONG Fang-li , WANG Xiao-lin* , SUN Xiao-归， WANG Jian-g扭g (Sch∞lofChemis住y and Phannaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China) Abstract: Objective: To establish methods for the determination of total flavonoids and hyperoside in leaves of Rosa davurica Pal l.. Methods: Total flavonoids were quantified spectrophotometrically at 418 nm using rutin as reference substance. For the determination of hyperoside by HPLC , the chromatogr叩hic s叩aration was performed on a Shim-pack VP-ODS C8 column (150 mm x 4.6 mm , 5 阳n) using a mobile phase composed of CH 0H-0.5% H P04 (45:55 , V/ηat 3 3 a flow rate of 1.0 mL/min , and the column tempera阳re and detection waveleng也 were 40 .C and 360 nm, respectively. Results: The line缸 ranges oftotal flavonoids and hyperoside were 0.02-0.12 mglmL (k =