电针足三里穴抗大鼠应激性胃黏膜损伤作用的中枢受体机制探讨.pdfVIP

480 咸宁学院学报(医学版)2007年第21卷第6期[Journal of Xianning College(Medical Sciences)] 电针足三里穴抗大鼠应激性胃黏膜 损伤作用的中枢受体机制探讨 刘寿仙 ，张 博 ，余 宙 ，王 盼 ，谈小琴 ，徐秀良 ，李彩伟 ，黄碧兰 (1．成宁摹院医学院生理学教研室，湖北成宁437100；2．成宁学院临床医学专业2003～2004级卓生) 摘要：目的探讨电针足三里穴抗大鼠应激性胃黏膜损伤作用及与中枢内受体关系。方法采用束缚-冷方 法制备大鼠应激性胃溃疡模型，观察孤束核(NTS)内微量注入不同受体阻断剂，胃溃疡指数(UI)的变化。结 果NTS内微量注射Or．．．受体阻断剂哌唑嗪、M．受体阻断剂阿托品均能削弱电针对大鼠应激性胃黏膜损伤保护 作用；给予(12-受体阻断剂育享宾、B．受体阻断剂心得安不影响电针的保护作用。结论 电针对大鼠应激性胃黏 膜损伤的保护作用部分是通过孤束核内Or．，、M受体介导的，而与Or．：、B受体无明显关系。 关键词：孤束核；受体；应激性胃黏膜损伤；电针；足三里穴 中图分类号：R245．9 文献标识码：A 文章编号：1008-0635(2007)06-0480-03 The Central Receptors Mechanism of Electroacupuncture at “Zusanli”Point against Stress Gastric Mucosa Injury LIU Shou-xian，ZHANG Bo，YU Zhou，et al (Department ofPhysiology，Xianning College，Xianning Hubei 437100，China) ABSTRACT：Objective To investigate the relationship between the electroacupuncture at‘‘Zusanli’’point against stress gastric mucosa injury and the central receptors．Methods The stress gastric ulcer model was es— tablished in rats by tie and cold method．The gastric ulcer indexes were observed after microinjection of various receptor antagonists into NTS．Results Microinjection of Otl receptor antagonist prazisin and M receptor antago— nist atropine both decreased the protective role of electroacupuncture against stress gastric mucosa injury in rats．Treatment with Ot2 receptor antagonist yohimbine and p receptor antagonist propanolol had no effect on the protective role of electroacupuncture．Conclusion The protective role of electroacupuncture against stress gastric mucosa injury in rats is partly mediated by Otl and M type receptors，and not related to Ot2 or p receptors． KEY WORDS：Nucleus tracms solitarius；Receptor；Stress gastric muCosa injury；Electroacupuncture； “Zusanli”point 应激性溃疡(stress ulcer)是临床危重急病的 团微量注射不同受体阻断剂，以探讨电针足