蛹虫草液体菌种培养和使用研究.pdfVIP

安徽农业科学．Journal of A』l}1ui A Sci．2007，35(36)：11908—11909 责任编辑 孙红忠 责任校对 李洪 蛹虫草液体菌种培养和使用研究 朱寿荣，温鲁 ，吴梅艳，姜春歌(淮阴师范学院资源微生物研究所，江苏淮安22330o) 摘要 [目的]明确碳氯源和培养方法对蛹虫草液体菌种的影响。[方法]配制不同碳氯源的培养液，接种蛹虫草菌种后分别进行振荡培 养和静置培养，观察培养液中茵丝球的大小、多少和分生孢子数量，再进行栽培比较：[结果]碳源以玉米糁、玉米、小麦、小麦米为好，氯 源以蛹汤和蛋白胨为好，培养方法则以振荡培养为好：[结论]选用适宜碳氮源进行蛹虫草的振荡培养，可获得优良的液体菌种。 关键词 蛹虫草；液体菌种；振荡培养；静置培养；茵丝球；分生孢子；代料栽培 中图分类号 Q936 文献标识码 A 文章编号 0517—6611(2007)36—11908—02 l／_esear~ 0llltivation and Use ofthe Bacterimn Growth Liquid of Cordyceps militaris 删 Simu．rong et al (ReSOUl~e and Microorganism Research Institute，Huaiyin Teachers College，Huaian，Jiangsu 2233(／0) A ih t In orderto clari the effect of carbon and nitrogen source and cultivationmethod onthe bacterium growsliquid of Cordycepsmilitaris sinensis． several culture Solutions were prepared with the different car~n and nitrogen sources．and inoculated th the bacterium grows liquid of Cordyceps rrdlitaris sinensis．Thenitwas cultivated bv oseillati~gand quietways separately． size，ntunber of h a ballandtl1P anaount ofmitogenic sporein cultureSolu— tionwere observed andthe cultivated methods were also compared．Theresuhindicatedthe better carbon SOUFCeweremaize，rice，andwheat grain；the bet— ter nitrogen source were pupa soup and peptone：and o~,illating cultivation was better than quiet cuhivation．111e conclusion was the excellent bacterium liq． uid of Cordyceps militaris sinensis Call be obtained with the proper carbon and nitrogen source and oscillating culti~ration i／lethod． Key words Cordyceps militaris；Bacterium grovel liquid；Oscillating cultivation；Quiet cuhivation；Hypha ball；Mitogenic spore 蛹虫草[Cordyceps militaris(L．)Link]，别名北冬虫夏草、北 2(】．00 g，磷酸二氢钾1．00 g，硫酸镁0．50 g，石膏1．00 g，补水 虫草，属麦角菌科(Clavicipitaceae)虫草属(Cordyceps)，蛹虫草 至750 ml，搅拌溶解，再加250 ml蛹汤(熬制方法见后)，分装 是该属的模式种_l J。已有许多研究认为蛹虫草可作为冬虫 l0个300ml三角瓶，每瓶100 ml。③玉米蛋白胨培养液：玉 夏草的替代品，其虫草素含量为冬虫夏草的数十倍 。虫草 米100．00 g，加500 ml自