雌激素受体亚型在妊娠期肝内胆汁淤积症患者胎盘的表达及意义.pdfVIP

现代妇产科进展2007年l2月第l6卷第l2期 Prog Obstet Gynecol，Dec．2007，Vo1．16，No．12 · 论著 雌激素受体亚型在妊娠期肝内胆汁淤积 症患者胎盘的表达及意义 罗 丹，刘淑云，邢爱耘 (四川大学华西第二医院妇产科，成都 610041) 【摘要】 目的：检测雌激素受体ot(estrogen receptor ot，ERa)、雌激素受体B(estrogen receptor B，ER13)在胎盘组织的表达，探讨ER与妊娠期肝内胆汁淤积症(intrahepatic cho— lestasis of pregnancy，ICP)的关系。方法：采用逆转录聚合酶链反应(reverse transcription— ploymerase chain reaction，RT—PCR)技术和免疫组化技术(SP法)检测胎盘ERa、ER13表 达。结果：ICP患者胎盘ER13在蛋白水平和mRNA水平表达均明显高于正常妊娠，差异 有统计学意义(P0．05)，ERa表达差异无统计学意义(P0．05)。结论：胎盘组织ERa 和ER13的蛋白水平与mRNA水平相一致，不存在蛋白翻译障碍；ICP可能与胎盘绒毛合 体滋养细胞ER13高表达有关。 【关键词】 妊娠期肝内胆汁淤积症；雌激素；雌激素受体ot、B；胎盘 - 中图分类号：R714．255文献标识码：A 文章编号：1004—7379(2007)12—091l—O3 ’I e expression and significance of estrogen receptor subtype on placenta of pregnant women with intrahepatic cholestasis． Dan，LiM Shuyun，Xing Aiyun．Department of Obstetrics and Gynecology， ￡ ina Second Hospital，Sichuan University，Chengdu 61004l 【Abstract】 Objective：To investigate the expression and the significance of ERa and ER13 in pregnant women with ICP．Method：The expressions of ERa and ER13 were investigated by reverse transcription—ploymerase chain reaction(RT—PCR)and immunhistochemistry(SP—as- say)．Results：The protein expression and the mRNA expression of ER13 on placenta villi of preg— nant women with ICP were significantly higher than those in normal pregnancy(PO．05)，which the protein and the mRNA expression of ERot were negative significance comparatively(P O．O5)．Condusion：The protein expression of ERot and ER13 on placenta are correlation with the mRNA expression of ERot and ER13．and there is no expression block of ERot and ER13．It shows ICP may be correlation with the higher expression of ER13． 【Key wards】Intrahepatic cholestasis of pregnancy；~ n；Estlx~n receptor仅、13；Placenta 妊娠期肝内胆汁淤积症(intrahepatic cholestasis 雌孕激素受体介导发挥其生物效应，故促使从雌激 of pregnancy，ICP)是一种重要的妊娠期并发症，早