鹅副粘病毒感染的诊断.pdfVIP

安徽农业科学，Journal ofAnhui A ．Sci．2007，35(36)：11837—11838 责任编辑 孙红忠 责任校对 李洪 鹅副粘病毒感染的诊断 钱晨 ，张建军2(1．扬州大学兽医学院，江苏扬州225oo9；2．江苏省扬州威克生物工程有限公司，江苏扬州225127) 摘要 [目的]为了确定江苏省某养鹅场20O7年春季发生传染病的病因。[方法]从发病鹅群采集病料进行流行病学调查、病原分离鉴 定、红血球凝集试验和红血球凝集抑制试验，通过交叉l皿试验测定 代分离株和Lasota毒株的l皿效价，并用稀释的F2代感染鸡胚尿囊 液分别感染鹅和SPF雏鸡，观察其发病和死亡情况。[结果]通过SPF鸡胚接种与传代从病、死鹅的脑、脾、胰混合病料分离到l株病毒。 经HA和l皿试验证明该分离株为禽I型副粘病毒。人工感染鸡和鹅试验表明该分离株对鸡和鹅都具有高致病性，同群混饲试验表明该 分离株具有很强的传染性，属强毒型NDV。这进一步证实该病毒属禽副粘病毒的新成员，即鹅副粘病毒I型。[结论]该研究为NDV出 现了某些新的致病特点以及其对鹅同样具有致病性提供了佐证。 关键词 鹅；病毒；HI；副粘病毒 中图分类号 $858．33 文献标识码 A 文章编号 0517—6611(20 )36一l1837—02 Diagnosis ofGoose Parmnyxovirus Infection QIANChela et al (College ofVeterinaryMedicine，Yangzhou University，Yangzhou，Jiangsu 225OO9) Abstract【Objective J e research aimedto confmnthe disensed cause ofinfectionthat occurredin a goosefarmin Jiangsu provincein Spring of2唧 ． [Method] e diseased samples were collected from the diseased gaggle to make epidemiological investigation，pathogen isolation and identification，hemag— glutination(HA)test and hemagglutination inhibition(HI)test， e l皿titers of F’iso1ates and Lasota virus strain were detennineel in cross l皿test． [he diluted allantoicfluid ofF2infected chicken embryowas usedtoinfect geeseand SPF chickento observethe diseaseand death conditions．【Resultl Bv SPF chicken embryo inoctdation and passage，one virus strain was iso1ated form the mixed diseased samples ofbrain，spleen and pancre3s ofdiseased and dead geese．1his isohated strain WaS proved to be avian patmr~ovims type I tk．,ugh HA and l皿lest．Artificial infection test on chicken and geese showed that this swain both had high pathogenicity to chicken and geese，and the test ofmixedfeedingin the SfllY~group showed that this isolate had strongerinfectivity and it belonged to virulent NDV，whieh~ther validated that this VilUS was a new member of avian pmamyxovirus，㈣ lv goose parmrtyxovirus．【Conclu- sion J is research provided evidences for the o