运动对慢性心理应激大鼠海马内皮素及其受体的影响.pdfVIP

文章编号：1000—677X(2007)12—0057—04 体育科学 2007年(第27卷)第12期 CHINA SP()RT SCIENCE Vo1．27，No．12，57—60，2007 运动对慢性心理应激大鼠海马内皮素及其受体的影响 Effects of Exercise on the Endothelin and its Receptor in Hippocampus of Chronic Psychological Stress Rats 于新凯 ，左 群 ，赛因·贺西格 ，孙庆文。，陆爱云 YU Xin—kai ，ZUO Qun ，SAIYIN He—xi—ge ，SUN Qing—wen ，LU Ai—yun 摘 要：目的：研究海马中内皮素(ET)及其受体(ET—R)在慢性心理应激过程中的变化和机 制，探讨运动对慢性心理应激大鼠海马内皮素及其受体的影响。方法：利用雄性SD大鼠建 立慢性心理应激动物模型，通过免疫组织化学和原位杂交法测定大鼠海马各区内皮素及其 受体的表达。结果：慢性心理应激过程中，随着应激程度的提高，海马各区ET含量逐渐下 降，ETmRNA的表达变化方向与ET一致，ET受体ETR—A含量则逐渐提高，运动可缓解以 上变化趋势。结论：慢性心理应激可影响海马各区ET及其受体的表达，实验所采用的游泳 运动模型可缓解慢性心理应激对海马的影响，但不能完全消除慢性心理应激的影响。 关键词：运动；心理应激；内皮素；受体；海马；鼠；动物实验 Abstract：The influence of exercise on the endothelin and its receptors in hippocampus of chron— ic psychological stress rats was discussed in this paper．The purpose was to explore the mean— ing and mechanism of the change aimut the endothelin and its receptor in hippocampus during the chronic psychological stress．The result showed that during chronic psychological stress stage，the content of ET decreased gradually in hippocampus，the same as the change of the E l mRNA．Conversely with the change of ET the content of rR—A in hippocampus increased gradually according to the stress leve1．It suggested that the express of ET and ET-R in hippo— campus could be changed by chronic psychological stress．The