甲鱼油对胰岛素抵抗细胞模型胰岛素敏感性和葡萄糖代谢的影响.pdfVIP

标记免疫分析与临床 2OO7年12月第14卷第4期 217 ● 基础研究 ● 甲鱼油对胰岛素抵抗细胞模型胰岛素敏感性 和葡萄糖代谢的影响 白 晶 ，田亚平 ，郭 多 (1．解放军总医院生化科，北京 100853；2．首都医科大学细胞生物学教研室，北京 100069) 摘要：采用高浓度胰岛素诱导建立胰岛素抵抗(IR)HepG 细胞模型，评价甲鱼油对 IR细胞模型胰岛素敏感性和 葡萄糖代谢的影响。利用甲鱼油处理IR细胞模型，通过葡萄糖氧化酶及。H一【)I葡萄糖参入实验分别检测其葡萄 糖消耗和。H一【)I葡萄糖参入率。结果表明，HepG 细胞模型的葡萄糖参入率在各种浓度的胰岛素刺激下均低于 对照细胞(PC0．05)，并且HepG 细胞模型与对照细胞的。H—I)I葡萄糖参入率随着胰岛素浓度的升高而升高，但 前者升高的幅度低于后者。MTT(甲基噻唑基四唑)结果显示甲鱼油均具有促进对照细胞和IR细胞模型增殖作 用。与对照组细胞相比，采用不同浓度胰岛素诱导的模型细胞经甲鱼油处理后其葡萄糖摄取和消耗显著增加(P O．05)。甲鱼油明显增加该 IR细胞模型的葡萄糖消耗和。H—I)_葡萄糖参入率，提高IR细胞模型的胰岛素敏感 性，改善其糖代谢。 关键词：甲鱼油； 胰岛素抵抗； 细胞模型； 胰岛素敏感性 中图分类号：R335． 6 文献标识码：A 文章编号：1006—1703(2007)04—0217-04 Effects of Turtle Oil on Insulin Sensitivity and Glucose Metabolism in Insulin Resistant Cell Model BAI Jing，TIAN Ya—ping，GUO DUO (Department of Clinical Biochemistry，General Hospitalof PI A．Beijing 100853．China) Abstract：To evaluate the effects of turtle oil on insulin sensitivity and glucose metabolism in an insulin—resistant(IR)cell model which was established by the way of high concentration of in— sulin induction with HepG2 cell in vitro culture．The IR cells were treated by turtle oil，the glu— cose consumption and H—D-glucose incorporation rate in IR cells were detected by the way of glucose oxidase and H—D-glucose incorporation assay respectively．The state of cell prolifera— tion was tested by MTT method．The results showed that the incorporation rate of。H—D-glu— cose in IR cells was significantly lower than that in the control cells(P~0．05)．The。H—D-glu— cose incorporation rate in either IR cells or control cells was increased with the increase of insu— lin concentration．Moreover，the。H—D-glucose incorporation