血必净注射液对慢性阻塞性肺疾病患者T淋巴细胞功能的影响.pdfVIP

主生垒 堂盘查 生 第1 鲞第12期Chin J EmergMed，Deceml：~r 2007，Vo1．16，No．12 ．1301． · 临床研究 · 血必净注射液对慢性阻塞性肺疾病患者 T淋巴细胞功能的影响 李旭 王立祥 张健鹏 李卉 刘亚华 【摘要】目的 通过观察血必净对慢性阻塞性肺疾病 (chronic obstructive pulmonary disease， COPD)患者T淋巴细胞增殖及分泌白细胞介素一2(IL一2)、表达CD25的影响，为血必净对COPD 的治疗提供理论依据。方法 采用酶联免疫吸附试验法测定血必净对T淋巴细胞分泌的细胞因子 IL广2影响，采用流式细胞仪测定血必净对T淋巴细胞表达CD25影响，用四甲基偶氨唑盐 (MTY)比 色法观察血必净对T淋巴细胞增殖的影响。结果 COPD患者T淋巴细胞生成的IL一2、表达CD25 水平与对照组比较显著减低 (P0．01)，T淋巴细胞增殖反应亦明显减低 (P0．01)；血必净和慢 阻肺组，PHA刺激T淋巴细胞，T淋巴细胞生成IL一2、表达CD25的较慢阻肺组高 (P0．01)，T 淋巴细胞增殖反应亦明显增加 (P0．01)。结论 COPD患者T淋巴细胞生成的IL一2、表达CD25 减低，T淋巴细胞增殖反应亦减低，免疫失衡；血必净促进COPD患者T淋巴细胞分泌IL一2、表达 CD25，增强T淋巴细胞增殖反应。 【关键词】肺疾病，慢性阻塞性；血必净；CD25；白细胞介素．2；T淋巴细胞 Effect of xuebijing on T lymphocyte fulton in paliens with chronic obstructive pulmonary~fiseases LI ， WANG Li-xiang，ZHANG Jian-peng，Li Hui，LIU Ya-hua． General Hospital of Chinese People’s Armed Police Forces，Being 100039，China Corresponding anther：L／Xu，Email：lixuangle@yahoo．con．cn 【Abatract】Objective Toobserve the effect of xuebijing on T lymphocyte function in patiens with acute exacerbation chronic obstructive pulmonary diseases(COPD)．Method IL一2 ofT lymphocytes in paUents with COPD were determined with the method of enzyme—linked immunoadsordent assay kits(ELISA)；the activation markers CD25 were analyzed by flow cytomery；proliferation assays of T lymphocytes were determined by MTr assay．Results In COPD patients ，IL一2 levels，the activation markers CD25，the prohferation assays were significantly lower than those ofnormal control group(P0．01)．We added xuebijing to PHA cultures ofT cells from COPD patients，IL一2 levels，the activation markers CD25 andthe proliferation assays T lymphocytes have increased in pared to PHA cultures of T cells from COPD group．C0I 叫坞 IL一2 levels，the activation markers CD25 and the proliferation assays ale abnormal in COPD patients。xuebijing Can enhance IL一2